Sequences from confirmation amplicon


Confirmation sequences for line 053D12 and hit At5g27330 are shown.
Line ID 053D12
Locus At5g27330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9633037
Primers used for PCR 05kh - 8474, show primer details
   
Sequence Name 40-K114240-0022-053-D12C-05kh
GenBank Accession LN503785 [GenBank]
Sequencing primer gene specific primer, 05kh: 5'-ATCAAGCCTAAGTGGAGACAAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K114240-0022-053-D12C-05kh
TTTGCAAATATATGAATATGAAACAACAAGTTTATTGGAGTTTCTGGAATCAACTACATG
GTTCTGAGAGAAAAGTAGCAGAAATCACAGACATAGAAGAGCTAGATACGAAAGGAAAAA
AGGTTCACATGATAGTTATTGAACCAACTACATAGAGGAGTTATGAGTAAAAAGCTCATA
AGCAACTACATAAATGGTTTATAAGTTAAACAGCGAAAATTGAGAACTCGAATGAGTTAA
AGGGTAGTACCATCTTCAGCTTCAGGTGATCACTTGAGAGAAGCAGCATAAGCAACAGAA
GCAGCCATGACAACAGAAGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 16-K114239-0022-053-D12C-8409
GenBank Accession LN493519 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K114239-0022-053-D12C-8409
CATTGAATATATCCTGCTTCTGTTGTCATGGCTGCTTCTGTTGCTTATGCTGCTTCTCTC
AAGTGATCACCTGAAGCTGAAGATGGTACTACCCTTTAACTCATTCGAGTTCTCAATTTT
CGCTGTTTAACTTATAAACCATTTATGTAGTTGCTTATGAGCTTTTTACTCATAACTCCT
CTATGTAGTTGGTTCAATAACTATCATGTGAACCTTTTTTCCTTTCGTATCTAGCTCTTC
TATGTCTGTGATTTCTGCTACTTTTCTCTCAGAACATGTAGTTGATTCCAGAAACTCCNA
TAAACTTGTTTGTTTCATATTCATATATNATGCAATATATATATGACATCTTTTGGTATC
AAGTTTTTGTCTCCACTAGGGCTTGATACCAAAA

Last Updated on 06.12.2018 16:11