Sequences from confirmation amplicon


Confirmation sequences for line 053F05 and hit At1g56580 are shown.
Line ID 053F05
Locus At1g56580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21198642
Primers used for PCR 27tg - 8474, show primer details
   
Sequence Name 42-K116770-0022-053-F05C-27tg
GenBank Accession LN504606 [GenBank]
Sequencing primer gene specific primer, 27tg: 5'-ATTGTGATGTTTCTGTTTTTGCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K116770-0022-053-F05C-27tg
ACTACTTTTTGTTGCTCAATTGTGCAACTTTTTTTAACATCCCAAGGTCCTTAAATCTTT
GACTTTATAACCATAAACCGTTGTAGGAGCTTTCATATTCTTACCCTAACACATGTCATT
AAATTTCATACAATGGCAAATTCCGTAATTAAATAGAGAATACAAGGGTGATTTCATACC
ACACCACCTATAAATTAAAAGGCAAAGATCTCCACAATAAACCTAAGAGACAGAGAGATC
TTAACACAAAACAAAGCAAACACCAAAAAAAACAGAGATGGGTTTGGTTACAGATGAAGT
GAGAGCTAGGGCAGAGAAATACACAGGAGATGAGATATGCCGTGAGAAGACAAAGGAGTT
TCTCAAGGAAGTTTCTATGCCTAATGGTTTATTGCCATTGAAGGACATTGAAGAAGTTGG
TTACGACAGAGAGACAGGTATTGTCTGGCTGAAGCAGAAGAAGAGCATCACCCACAAGTT
TGAAGCCATTGGTAAACTTGTCTCTTACGCCACCGAGGTCATTGCACAGGTGGAGGTCAT
GAAGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCG
   
Sequence Name 18-K116769-0022-053-F05C-8409
GenBank Accession LN494473 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K116769-0022-053-F05C-8409
ATCCTGCTTCATGACCTCCACCTGTGCAATGACCTCGGTGGCGTAAGAGACAAGTTTACC
AATGGCTTCAAACTTGTGGGTGATGCTCTTCTTCTGCTTCAGCCAGACAATACCTGTCTC
TCTGTCGTAACCAACTTCTTCAATGTCCTTCAATGGCAATAAACCATTAGGCATAGAAAC
TTCCTTGAGAAACTCCTTTGTCTTCTCACGGCATATCTCATCTCCTGTGTATTTCTCTGC
CCTAGCTCTCACTTCATCTGTAACCAAACCCATCTCTGTTTTTTTTGGTGTTTGCTTTGT
TTTGTGTTAAGATCTCTCTGTCTCTTAGGTTTATTGTGGAGATCTTTGCCTTTTAATTTA
TAGGTGGTGTGGTATGAAATCACCCTTGTATTCTCTATTTAATTACGGAATTTGCCATTG
TATGAAATTTAATGACATGTGTTAGGGTAAGAATATGAAAGCTCCTACAACGGTTTATGG
TTATAAAGTCAAAGATTTAAGGACCTTGGGATGTTAAAAAAAGTTGCACAATTTGAGCAA
CAAAAAGTAGTTGATCATTGCCTTAAGTGTGAGTCTTTTAGTTTTAAAATTCAGTCTGCA
AAAACAGAAACATCACAA

Last Updated on 06.12.2018 16:11