Sequences from confirmation amplicon


Confirmation sequences for line 053G03 and hit At1g04180 are shown.
Line ID 053G03
Locus At1g04180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1105486
Primers used for PCR PH77 - 8474, show primer details
   
Sequence Name 33-K017511-022-053-G03C-PH77
GenBank Accession LN500795 [GenBank]
Sequencing primer gene specific primer, PH77: 5'-CTTCTGTCGTTTGGTCTCTTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K017511-022-053-G03C-PH77
CTTGAGCGATGCTAACGGCGTCTACTGATGCTCCAGCCAACCCATTTTCTCGTGAATCCG
GCCGCGTATAATCCCGATTTCCCTTTCCACGCGTTTGGAAACGGCGATTTCGGAAACCCA
TTTTTCGAAAAGAACTCACTTTACTGATGACGAAAATGGACGTTGCTACGATAACCGGTG
GGGAGAACCACAGAGCCAATATCTAGCTTCTGACCATCTACGAGCTCCACGTGGTGACGC
GAGAACTGTTTGATTGCAGGTACAATTTCTACGTCGCCGGATTTTATCTTCTCCAGAGCA
CCGATGTCAAGAACCGGCGTCTTTCCTGTCATGCTTTTAAGCTCCATTGGGCCGATGTCA
GGCCTTTTAAGCCCCTACTCTGATAAGCTCCCTAGAACCAACCACGATAAGATAAGCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTT
ATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
ATTC
   
Sequence Name 37-K017511-022-053-G03C-8409
GenBank Accession LN502437 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K017511-022-053-G03C-8409
GATTTCCCGGACATGAAGCCATTTACAATTGCTTATCTTATCGTGGTTGGTTCTAGGGAG
CTTATCAAACTATGGGCTTAAAAGGCCTGACATCGGCCCAATGGAGCTTAAAAGCATGAC
AGGAAAGACGCCGGTTCTTGACATCGGTGCTCTGGAGAAGATAAAATCCGGCGACGTAGA
AATTGTACCTGCAATCAAACAGTTCTCGCGTCACCACGTGGAGCTCGTAGATGGTCAGAA
GCTAGATATCGACGCTGTGGTTCTCGCCACCGGTTATCGTAGCAACGTCCCTTCTTGGCT
TCAGGAAAGTGAGTTCTTTTCGAAAAATGGGTTTCCGAAATCGCCGTTTCCAAACGCGTG
GAAAGGGAAATCGGGATTATACGCGGCCGGATTCACGAGAAAAGGGTTGGCTGGAGCATC
AGTAGACGCCGTTAACATCGCTCAAGACATTGGAAATGTGTGGAGAGAAGAGACCAAACG
ACAGAA

Last Updated on 06.12.2018 16:11