Sequences from confirmation amplicon


Confirmation sequences for line 053G06 and hit At1g35510 are shown.
Line ID 053G06
Locus At1g35510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:13074447
Primers used for PCR MR96 - 8474, show primer details
   
Sequence Name 49-K017449-022-053-G06C-MR96
GenBank Accession LN506670 [GenBank]
Sequencing primer gene specific primer, MR96: 5'-GGGTGAGAATGTTGAAATGTTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K017449-022-053-G06C-MR96
ATAGCTTTTAAAGTTCTGTCTTTTTAGAAGCCCTGTTTATAGGAAAGAGAAATTTATGTC
CACTGGTTGGTAGGAATCAGAATGATTCTATTATTAGGTTGGGAGCGTGTTATGTTCTTA
AAGCAATTTGCCCATAAAACCCGTGGGCTCTCCCATAACATGCCCTTGGAATAGAAAGAT
TTTGACCATTATATAATATTCTAGTTCACTAAACAAATTGCTGTATTTGTGAAACTGTAG
GGACACTCATCAAGATTGGCAGCACTTGATTACACAGTATGCCTCCACAGTGAAGTGTTT
GTATCCACTCAAGGTGGAAACTTCCCACATTTCTTGATAGGTCACCGTCGCTACCTTTAC
AAAGGCCACGCTGAGACAATCAATTTGTATGAACATTGCCGAGCGCATCCAGGAGGCCGG
CGCGGGCCTGCGTAGCCTGGCAGAGCCGTGGGCCGACACCACCACGCCGGCCGGCCGCAT
GGTGTTGACCGTGTTCGCCGGCATTGCCGAGTTCGAGCGTAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACA
TTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATT
ATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
   
Sequence Name 50-K017449-022-053-G06C-8409
GenBank Accession LN507019 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K017449-022-053-G06C-8409
TCCATGTAGATTTCCCGGACATGAAGCCATTACGCTCGAACTCGGCAATGCCGGCGAACA
CGGTCAACACCATGCGGCCGGCCGGCGTGGTGGTGTCGGCCCACGGCTCTGCCAGGCTAC
GCAGGCCCGCGCCGGCCTCCTGGATGCGCTCGGCAATGTTCATACAAATTGATTGTCTCA
GCGTGGCCTTTGTAAAGGTAGCGACGGTGACCTATCAAGAAATGTGGGAAGTTTCCACCT
TGAGTGGATACAAACACTTCACTGTGGAGGCATACTGTGTAATCAAGTGCTGCCAATCTT
GATGAGTGTCCCTACAGTTTCACAAATACAGCAATTTGTTTAGTGAACTAGAATATTATA
TAATGGTCAAAATCTTTCTATTCCAAGGGCATGTTATGGGAGAGCCCACGGGTTTTATGG
GCAAATTGCTTTAAGAACCTAACACGCTCCCAACCTAATAATAGAATCATTCTGATTCCT
ACCAACCAGTGGACATAAATTTCTCTTTCCTATAAACAGGGCTTCTAAAAAGACAGAACT
TTAAAAGCTATCATAAAAACATTTCA
Primers used for PCR FM16 - 8474, show primer details
   
Sequence Name 13-K029742-022-053-G06C-8409
GenBank Accession LN491907 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K029742-022-053-G06C-8409
GCCATTTACAATTGAATATATCCTGGCCGNNCCGCTGCCGCTTTGCACCCNGGTGGAAGC
TTGNNCATGTTGGTTTCTACGCAGAACTGAGCCGGTTAGGCAGATAATTTCCATTGACAT
CAAGCTCTTAGATTCCTCTGCCTCTAAAGCATTTTCTTCGGTTTGGACGTTTTTTTTTTT
TGTAGAGGGGACTACTTCAAAAAACAAATGCAAAATATGCTTAAACACAATGATGCAAAG
GGAGTCAAATTAAGGAAACCCGCTGCATCACTCTACACGTTCCCCATGCCCAACTGCATG
TGTAAAAAACCCAATCCGGAACCTGAAACCGATCCAGCTAAAACCGGATTCCTGCTGTTC
AGGGTTAATTATTGTTTATGACTTCAACCATACATGTAACTTATCTATATATATGTGGTA
TCAGGAGCCCGGAAAAAGCATTTTTCCCCTTCAAAAGTGGGGGGAATCTAAAATTAGTAT
TTTTTTATTGATACAAATTTTTGTGTGAACATAACATTCTTTCAACATTAGGGACC
   
Sequence Name 14-K029742-022-053-G06C-FM16
GenBank Accession LN492378 [GenBank]
Sequencing primer gene specific primer, FM16: 5'-AAGGGAGAATGGTCACTAATGTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K029742-022-053-G06C-FM16
CAAATTCGTATCAATAAAAAAATACTAATCTTAGATTCCCCCAACTTTTGAAGGGGAAAA
ATGCTTATACAGGACTCATGATACAACATATATATAGATAAGTTACATGTATGGTTGAAG
TCATAAACAATAATTAACACTGAACAGCAGGAATCCGGTTTAAGCTGGATCGGTTTCAGG
TTCCGGATCGGGTTCTTTACACATGCAGTCGGGCATGGGGAACGTGTAGAGTGATGCAGC
GGGTTTCCTTAATTCGACTCCCTTTGCATCATTGTGTCTAAGCATATCTTGCATTTGTTT
CTTGAAGTAGTCCCATCTACAAAAAAAAAAAACGTCCAACCCAAAAAAAATGCTTTAAAG
GCAAAGAAATCTAAAAGCTTGATGTCAATGGAAATTATCTGCCTAACCGGCTCATTTCTG
CGTAAAAACCAACATGCAAGCTCCCCCGGGTGCAAAGCGGCACCGGCGGCAGGATATATT
CAATTGAAAATGGCTTCATGTCCGGAAAATCTACTTGGATCACCAATGAGTATGATGGTC
AATATGGAAAAAAAAAAAGAGTAATTCCCAATTTTTTTTCAATTCAAAAATGTAAATGTC
CCCACCGTTTTTATAAAATGAAAGTACTTTTTGATA

Last Updated on 06.12.2018 16:11