Sequences from confirmation amplicon


Confirmation sequences for line 053G07 and hit At5g02800 are shown.
Line ID 053G07
Locus At5g02800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:636662
Primers used for PCR DL15 - 8474, show primer details
   
Sequence Name 03-K018611-022-053-G07C-DL15
GenBank Accession LN487283 [GenBank]
Sequencing primer gene specific primer, DL15: 5'-TTTTGTCATGCAGATACTCCAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K018611-022-053-G07C-DL15
CCACCGGCAGCTATTTTCATTCTGGTGTTCCAGTCAAGTGGCTGCTTACCAGGTGAAATG
TCTGAAATAAAAAAAGCATCACCCGATTGAAATTTAAAGACTTCTCATAGGCATGTGTGA
TCTTAGAAGCGCATACCGTGCAAATGATCTTCAAGTGAACCTAGGGGCATGTACTCGTAG
ACTAGAAGCCTTTGATCTCCATCAGCACAATATCCTATTAAGTTAACAAGATTCGGGTGA
TGAAGAAGACTCAACATGAGAACCTCGACGAGGAACTCCCTGTTTCCTTGTAAACCATTA
TGATCAAGTTGCTTGATAGCCGCTGTCTACAAATGAAGATATAATGACAGTGAGCTTTCA
CTACCAAGAAGATTCAAAAGGTTAAAACCAAGCAAAAAAAAGGGAAGGAACCTGGCTAGT
GCTGGCCAAGTATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGA
CAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAA
ATCTTTATTTCGACGTGTCT
   
Sequence Name 04-K018611-022-053-G07C-8409
GenBank Accession LN487726 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K018611-022-053-G07C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATACTTGGCCAGCACTAGCCAG
GTTCCTTCCCTTTTTTTTGCTTGGTTTTAACCTTTTGAATCTTCTTGGTAGTGAAAGCTC
ACTGTCATTATATCTTCATTTGTAGACAGCGGCTATCAAGCAACTTGATCATAATGGTTT
ACAAGGAAACAGGGAGTTCCTCGTCGAGGTTCTCATGTTGAGTCTTCTTCATCACCCGAA
TCTTGTTAACTTAATAGGATATTGTGCTGATGGAGATCAAAGGCTTCTAGTCTACGAGTA
CATGCCCCTAGGTTCACTTGAAGATCATTTGCACGGTATGCGCTTCTAAGATCACACATG
CCTATGAGAAGTCTTTAAATTTCAATCGGGTGATGCTTTTTTTATTTCAGACATTTCACC
TGGTAAGCAGCCACTTGACTGGAACACCAGAATGAAAATAGCTGCCGGTGCTGCAAAAGG
TTTGGAGTATCTG
Primers used for PCR FM17 - 8474, show primer details
   
Sequence Name 33-K029300-022-053-G07C-8409
GenBank Accession LN500872 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K029300-022-053-G07C-8409
ATTGAATATATACTTGGCCAGCACTAGCCAGGTTCCTTCCCTTTTTTTTGCTTGGTTTTA
ACCTTTTGAATCTTCTTGGTAGTGAAAGCTCACTGTCATTATATCTTCATTTGTAGACAG
CGGCTATCAAGCAACTTGATCATAATGGTTTACAAGGAAACAGGGAGTTCCTCGTCGAGG
TTCTCATGTTGAGTCTTCTTCATCACCCGAATCTTGTTAACTTAATAGGATATTGTGCTG
ATGGAGATCAAAGGCTTCTAGTCTACGAGTACATGCCCCTAGGTTCACTTGAAGATCATT
TGCACGGTATGCGCTTCTAAGATCACACATGCCTATGAGAAGTCTTTAAATTTCAATCGG
GTGATGCTTTTTTTATTTCAGACATTTCACCTGGTAAGCAGCCACTTGACTGGAACACCA
GAATGAAAATAGCTGCCGGTGCTGCAAAAGGTTTGGAGTAT
   
Sequence Name 34-K029300-022-053-G07C-FM17
GenBank Accession LN501289 [GenBank]
Sequencing primer gene specific primer, FM17: 5'-TTTTGTCATGCAGATACTCCAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K029300-022-053-G07C-FM17
TTTTCTTCTGGTGTTCCANTCAAGTGGCTGCTTACCAGGTGAAATGTCTGAAATAAAAAA
AGCATCACCCGATTGAAATTTAAAGACTTCTCATAGGCATGTGTGATCTTAGAAGCGCAT
ACCGTGCAAATGATCTTCAAGTGAACCTAGGGGCATGTACTCGTAGACTAGAAGCCTTTG
ATCTCCATCAGCACAATATCCTATTAAGTTAACAAGATTCGGGTGATGAAGAAGACTCAA
CATGAGAACCTCGACGAGGAACTCCCTGTTTCCTTGTAAACCATTATGATCAAGTTGCTT
GATAGCCGCTGTCTACAAATGAAGATATAATGACAGTGAGCTTTCACTACCAAGAAGATT
CAAAAGGTTAAAACCAAGCAAAAAAAAGGGAAGGAACCTGGCTAGTGCTGGCCAAGTATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCG
TCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGAC
GTGTCTACATT

Last Updated on 06.12.2018 16:11