Sequences from confirmation amplicon


Confirmation sequences for line 053H04 and hit At5g35160 are shown.
Line ID 053H04
Locus At5g35160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:13416004
Primers used for PCR 05lu - 8474, show primer details
   
Sequence Name 54-K113947-0022-053-H04C-05lu
GenBank Accession LN508547 [GenBank]
Sequencing primer gene specific primer, 05lu: 5'-CAGATGCTGGACATGATAAAGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K113947-0022-053-H04C-05lu
CGCCAGAACCAGAAGCCAGGATGGGTATTTCTGAGCGGGGATTTCCCGGGGAATTTGGTT
TGTTCGAACTGGGAATTCAATGTGAGGAGCCTTGGCTCCAAAGTAACCACCGATAAGAGT
TAGAGGAACCGAGATGCAAAACCACAGGAGGAGGAGGATGACAAATAGAGAGAAAGGAAT
GGCTCCAGTGCTATGGCTACCCCATAGAAGGAAGTTCAGCGTTGTAAGGATCAGAAACGC
AATACCCGGGAAAAAGCAAGCAGCTTTCCATGCGACAGACATCCATCCACGGTGTTCGCC
ACAACCAATTGTCCTCCAAAGACGAACAGAAACATAACCAGCTGCAATGCCAAGAATCAT
GTAGAAGAAGAGCATACCAGTAATAAGTGTTCCACGTGAAGCCGGTGACATGAACCCTAG
AGCAGCAAACAAAATGGTAACAACAGCCATGCCAAGAATCTGAACTCCGTCACCAACCAT
GACACACAATAGCGAGGCATTTGATGGAGCACGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCC
   
Sequence Name 26-K113946-0022-053-H04C-8409
GenBank Accession LN498020 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K113946-0022-053-H04C-8409
CAATGCCTCGCTTTGTGTGTCATGGTTGGTGACGGAGTTCAGATTCTTGGCATGGCTGTT
GTTACCATTTTGTTTGCTGCTCTAGGGTTCATGTCACCGGCTTCACGTGGAACACTTATT
ACTGGTATGCTCTTCTTCTACATGATTCTTGGCATTGCAGCTGGTTATGTTTCTGTTCGT
CTTTGGAGGACAATTGGTTGTGGCGAACACCGTGGATGGATGTCTGTCGCATGGAAAGCT
GCTTGCTTTTTCCCGGGTATTGCGTTTCTGATCCTTACAACGCTGAACTTCCTTCTATGG
GGTAGCCATAGCACTGGAGCCATTCCTTTCTCTCTATTTGTCATCCTCCTCCTCCTGTGG
TTTTGCATCTCGGTTCCTCTAACTCTTATCGGTGGTTACTTTGGAGCCAAGGCTCCTCAC
ATTGAATTCCCAGTTCGAACAAACCAAATTCCCCGGGAAATCCCCGCTCAGAAATACCCA
TCCTGGCTTCTGGTTCTTGGCGCTGGAACACTTCCATTCGGTACCCTCTTCATCGAGCTT
TTCTTTATCATGTCAGGCATCT

Last Updated on 06.12.2018 16:11