Sequences from confirmation amplicon


Confirmation sequences for line 054A01 and hit At1g06260 are shown.
Line ID 054A01
Locus At1g06260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1916742
Primers used for PCR 03sa - 3144, show primer details
   
Sequence Name 16-K116188-0022-054-A01C-03sa
GenBank Accession LN493577 [GenBank]
Sequencing primer gene specific primer, 03sa: 5'-CCAGTACTTTTGATCGCCTTCTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K116188-0022-054-A01C-03sa
AATTTGTGAAAACACCGGAGGAGTAGAGCTGAAAGATAAACCCACCAGCATCGATTCCAA
CTGATACTGGCTGTTGAGCTGCAGCGATTTGTAGGCTCGCCTCGTTTTGGGCTACCTTTT
GGTATCCTTGTATCGTAACTACCTTATTTTTGGATTTTTCTTGGTCGCAGGTACCCTCTA
TGCCTGTGTATGGGTAGTCGGTCTCCGTGGCGAGTCCACCATTGGTTTTGATGAATTCAA
ATGCTGTTTCCATTAATCCGCCACTACAGCCTTTGTTGTAAGTGCCGACATCACAATCTA
TGAGTTGTTGTTCTGAGAGAGACACTAAATTCCCTGTTTTAATCTTGTTGATGCCTTCAA
TAGCTGCTACTGCAGAGAATGCCCAGCAACCTCCTGCAACAAGAAAAACAAAACGCAATC
AGAATGTCATCAGTTGAATAATGAATGTTTGCAAGGTTTTATGTTTAGAATGAATTATGT
AAAAACATAATTTCATACCGCATTTTCCTTGATTTCTGATAGGAGTCACAGCACCTTGAG
TCCTCCAGTCCACAGCGTCCGGGACATTGCCTGCTGGGTCACAAACTGGTCTCTGCTTCT
TGTGTAACCTCAAGGAAGACGTGTTCAACCCGAGAAAATGGGCCTTAAACCGAAGGCGGG
AAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATAT
AGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACATCAGTGGAGAT
ATCA
   
Sequence Name 06-K116187-0022-054-A01C-3144
GenBank Accession LN488962 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K116187-0022-054-A01C-3144
CAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGAT
CAGATTGTCGTTTCCCGCCTTCGGTTTAAGGCCCATTTTCTCGGGTTGAACACGTCTTCC
TTGAGGTTACACAAGAAGCAGAGACCAGTTTGTGACCCAGCAGGCAATGTCCCGGACGCT
GTGGACTGGAGGACTCAAGGTGCTGTGACTCCTATCAGAAATCAAGGAAAATGCGGTATG
AAATTATGTTTTTACATAATTCATTCTAAACATAAAACCTTGCAAACATTCATTATTCAA
CTGATGACATTCTGATTGCGTTTTGTTTTTCTTGTTGCAGGAGGTTGCTGGGCATTCTCT
GCAGTAGCAGCTATTGAAGGCATCAACAAGATTAAAACAGGGAATTTAGTGTCTCTCTCA
GAACAACAACTCATAGATTGTGATGTCGGCACTTACAACAAAGGCTGTAGTGGCGGATTA
ATGGAAACAGCATTTGAATTCATCAAAACCAATGGTGGACTCGCCACGGAGACCGACTAC
CCATACACAGGCATAGAGGGTACCTGCGACCAAGAAAAATCCAAAAATAAGGTAGTTACG
ATACAAGGATACCAAAAGGTAGCCCAAAACGAGGCGAGCCTACAAATCGCTGCAGCTCAA
CAGCCAGTATCAGTTGGAATCGATGCTGGTGGGTTTATCTTTCAGCTCTACTCCTCCGGT
GTTTTCACAAATTACTGTGGAACAAATCTCAACCATGGAGTGACTGTTGTTGGATATGGA
GTAG

Last Updated on 06.12.2018 16:11