Sequences from confirmation amplicon


Confirmation sequences for line 054A02 and hit At1g30760 are shown.
Line ID 054A02
Locus At1g30760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10919341
Primers used for PCR 74zk - 8474, show primer details
   
Sequence Name 45-K129065-0022-054-A02C-74zk
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 74zk: 5'-AACTATACATTTCCCTCATCCACC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K129065-0022-054-A02C-74zk
GTCAGCCATTGTATCTTAGAACAATGTCCCACTCCTATGTGGGAAAGGTGTCTCTGTCTC
GGGAATTTTTGCCATCATTCCTCCATAAGGATTCCATATAGTCAATGGTGAGTCCTCTTC
TAGAAGCTTCTCCCACAACCCTTCTAATCCCTCTACTGGAATTGGCTCTTCCACATAGTC
TGACTTGGCTTTGAAGTAATTCTTGAACAACGATTTCCCATCGAGTAAAGCTTCTGATGG
GGCAGTGCTTGGAAAACCTGCAATGTACATCACCGATTTGATCCAGCTTGTCTCTAAACA
ATCTTTCTTCGTTAGTCCAAGCTGTGGGAAACTCCTTTGCATCACCTGTCAAACAAACCC
GGGGTTGTTATTGTCATAATCAAAACTTTCCTTCCTTAATTTATCATAACCATTAACTTT
CATAAATTAACAAACAGACCCCTAATATTACGAAATTTAAATTTGACCCCATTAATTTTA
CCTGCAACAAACGATTAGCGTCGCCGAGAAATTGGCCTTGGTACGAAGTCGAGATAGTTC
TTTCTTTGCTCTTCGGAGTTTTGCTCGTCGGCTGAATAATCACACGGATAAAGAGATCTT
CGTCGAGCTTATCAGCGACTTGTTGCCACTTGTACAAAAGCTTAGTTCCGTCTTGCTCAA
GCGTCCTCGTGACGGTAAACACCGTAACAATCTCTGGCACCGGAACGAGCTTTATTTTCC
AGGCAAGTATTACGCCGAAGCTTCCTCCTCCACCTCCTCGAATCGCCCAGAAAACGTCTT
CTCCCATCGCCGCGCGATTCAAGATTTTGCCGTCAGCGTCAACGACATGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGGAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAATGT
   
Sequence Name 03-K129064-0022-054-A02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K129064-0022-054-A02C-8409
TTTCATTGAATATATCATGTCGTTGACGCTGACGGCAAAATCTTGAATCGCGCGGCGATG
GGAGAAGACGTTTTCTGGGCGATTCGAGGAGGTGGAGGAGGAAGCTTCGGCGTAATACTT
GCCTGGAAAATAAAGCTCGTTCCGGTGCCAGAGATTGTTACGGTGTTTACCGTCACGAGG
ACGCTTGAGCAAGACGGAACTAAGCTTTTGTACAAGTGGCAACAAGTCGCTGATAAGCTC
GACGAAGATCTCTTTATCCGTGTGATTATTCAGCCGACGAGCAAAACTCCGAAGAGCAAA
GAAAGAACTATCTCGACTTCGTACCAAGGCCAATTTCTCGGCGACGCTAATCGTTTGTTG
CAGGTAAAATTAATGGGGTCAAATTTAAATTTCGTAATATTAGGGGTCTGTTTGTTAATT
TATGAAAGTTAATGGTTATGATAAATTAAGGAAGGAAAGTTTTGATTATGACAATAACAA
CCCCGGGTTTGTTTGACAGGTGATGCAAAGGAGTTTCCCACAGCTTGGACTAACGAAGAA
AGATTGTTTAGAGACAAGCTGGATCAAATCGGTGATGTACATTGCAGGTTTTCCAAGCAC
TGCCCCATCAGAAGCTTTACTCGATGGGAAATCGTTGTTCAAGAATTACTTCAAAGCCAA
GTCAGACTATGTGGAAGAGCCAATTCCAGTAGAGGGATTAGAAGGGTTGTGGGAGAAGCT
TCTAGAAGAGGACTCACCATTGACTATATGGAATCCTTATGGAGGAATGATGGCAAAAAT
TCCCGAGACAGAGACACCTTTCCCACATAGGAGTGGGACATTGTTCAAGATACAATGGCT
GACTTTGTGGCAAGATGGGAAAACAAGCGAGGCGAAGCATATGGGGTGGATG

Last Updated on 06.12.2018 16:11