Sequences from confirmation amplicon


Confirmation sequences for line 054A04 and hit At1g67330 are shown.
Line ID 054A04
Locus At1g67330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25214708
Primers used for PCR 01gw - 8474, show primer details
   
Sequence Name 23-K112307-0022-054-A04C-01gw
GenBank Accession LN496618 [GenBank]
Sequencing primer gene specific primer, 01gw: 5'-CTTAAGATGATGCCAATCCAATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K112307-0022-054-A04C-01gw
ATCAGATATTTTTTCTTAACTTGTATTTTTTAATAGGCAAATGTAGAAGCTAGGCCTCTT
AAAACATTATGAAACAAAGAGTAATAACGATAGAATACATAATTTATTTTAATGAGAAGC
CTCTGGATATAGTTTATATTTTAATAACGATTTTTAAACTCAGATCGAACTATGCGGTGC
TCCAATTAAAAGAAGTATCGAAGAACGAATATTTGGCGCACTCTAGCAAAACCGGTCACC
AGGCTGGTCGGTCATGTTAGCGGCGTTAGGTATCTCGAAGTGCCAGAGCCTACCTACGGA
GTTGACCTTATACTTCTCACAAAGGAACTCATTGGCAAAAGCGTTCTCCACTTTGCGGTT
AACGTCATGAAGGAAGACGTGAGTCGTGCCATCACCTTTCCGGTTACGTGCCATGATGGC
CGAGGAAAATATCGCCGCCATCCTTCCTGGCGCCTCTGGGAAGTACCCTTTTGGTGCGTC
CACCATGATCAGATCCCACTCGGTATCATAGAACTCATCAGTAATGAGTAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 07-K112306-0022-054-A04C-8409
GenBank Accession LN489339 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K112306-0022-054-A04C-8409
TGATACCGAGTGGGATCTGATCATGGTGGACGCACCAAAAGGGTACTTCCCAGAGGCGCC
AGGAAGGATGGCGGCGATATTTTCCTCGGCCATCATGGCACGTAACCGGAAAGGTGATGG
CACGACTCACGTCTTCCTTCATGACGTTAACCGCAAAGTGGAGAACGCTTTTGCCAATGA
GTTCCTTTGTGAGAAGTATAAGGTCAACTCCGTAGGTAGGCTCTGGCACTTCGAGATACC
TAACGCCGCTAACATGACCGACCAGCCTGGTGACCGGTTTTGCTAGAGTGCGCCAAATAT
TCGTTCTTCGATACTTCTTTTAATTGGAGCACCGCATAGTTCGATCTGAGTTTAAAAATC
GTTATTAAAATATAAACTATATCCAGAGGCTTCTCATTAAAATAAATTATGTATTCTATC
GTTATTACTCTTTGTTTCATAATGTTTTAAGAGGCCTAGCTTCTACATTTGCCTATTTAA
AAAATACAAGTATAAGAAAAAATTTATCTGGATTTAACTTGTGCTACACAAATTGGATTG
GCTCAATCTTAA

Last Updated on 06.12.2018 16:11