Sequences from confirmation amplicon


Confirmation sequences for line 054B01 and hit F21M11 are shown.
Line ID 054B01
Locus F21M11 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1022130
Primers used for PCR 03of - 8474, show primer details
   
Sequence Name 24-K114720-0022-054-B01C-03of
GenBank Accession LN497104 [GenBank]
Sequencing primer gene specific primer, 03of: 5'-CAACGTCTGATGCAGGATATCTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K114720-0022-054-B01C-03of
GGTCACAGGGAAATTCAATTAAGCTAGCTACAGTGCTGGTTCTTGTGAGGATTTGTTACC
TATTTTATTTGATTAGTTTAAAACCAACGTGCCTTCATGGCTTCAGAATAAAATAAAATA
AACAATTTCCCCCAGTTGCAATAAAGAAAAAACAGTAGGTGAGTCTTAGTCAAGGTCAAT
TAGTTTTCAAATCAATAATTATTTTCCAAACCGATCCAAACCGGCTAAATAACCATCAGT
CTCCGCGGTTTAAGATTTGACTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 04-K114719-0022-054-B01C-8409
GenBank Accession LN488021 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K114719-0022-054-B01C-8409
TCAAATCCTTAAACCGCGGAGACTGATGGTTATTTAGCCGGTTTGGATCGGTTTGGAAAA
TAATTATTGATTTGAAAACTAATTGACCTTGACTAAGACTCACCTACTGGTTTTTTCTTT
ATTGCAACTGGGGAAATTGTTTATTTTATTTTATTCTGAAGCCATGAAGGCACGTTGGTT
TTAAACTAATCAAATAAAATAGGTAACAAATCCTCACAAGAACCAGCACTGTAGCTAGCT
TAATTGAATTTCCCTAGTAACCTATGTTATTTATTTTGATGCAAATTTTTATCGTCTTAA
GTTAGATATCCTGC

Last Updated on 06.12.2018 16:11