Sequences from confirmation amplicon


Confirmation sequences for line 054C08 and hit T4L20 are shown.
Line ID 054C08
Locus T4L20 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16530295
Primers used for PCR JU02 - 8474, show primer details
   
Sequence Name 07-K019769-022-054-C08C-JU02
GenBank Accession LN489058 [GenBank]
Sequencing primer gene specific primer, JU02: 5'-AGGACAATGTTGTGGCTATGTCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K019769-022-054-C08C-JU02
TTTTCCGGGCTGGATATTTACGAGAATGCCATCCCGGGAGATGAAATGGAGTATGTAAAC
CCCGGAAGTCGCCAGAACCGGATAAATTCTTCACAATTGGTACATGATTTTGTAGCTTGA
AGAAGTAAGCAATGGAGAGGAATAAAGCATAGGTGTGATGAACTTATGATCAGTCTCAAT
TCTGTAGTGGAAGAATGTTGGTCAGAAGAAAAAGTTTTAGAAATATTTGTATAAAGTAAA
CATTCTTTTGTACAAGAATATAAGTGAGGTTAGGTCATTGGGACCAAACAGAATATGGTA
AATGCAATTTGAGATCTGCAAGAGATGGACATTACACTGTAGACTTATTATCTTATCACA
TCTACATGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAA
ATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCA
ATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 08-K019769-022-054-C08C-8409
GenBank Accession LN489525 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K019769-022-054-C08C-8409
TAGATTTCCATGTAGATGTGATAAGATAATAAGTCTACAGTGTAATGTCCATCTCTTGCA
GATCTCAAATTGCATTTACCATATTCTGTTTGGTCCCAATGACCTAACCTCACTTATATT
CTTGTACAAAAGAATGTTTACTTTATACAAATATTTCTAAAACTTTTTCTTCTGACCAAC
ATTCTTCCACTACAGAATTGAGACTGATCATAAGTTCATCACACCTATGCTTTATTCCTC
TCCATTGCTTACTTCTTCAAGCTACAAAATCATGTACCAATTGTGAAGAATTTATCCGGT
TCTGGCGACTTCCGGGGTTTACATACTCCATTTCATCTCCCGGGATGGCATTCTCGTAAA
TATCCACCCCGGAAAAGTTGTTGTATGCCTCACTAGACATAGCCACAACATTGTCC
Primers used for PCR FM19 - 8474, show primer details
   
Sequence Name 67-K029747-022-054-C08C-8409
GenBank Accession LN511727 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K029747-022-054-C08C-8409
GCCATTTACAATTGACCATCATACTCATTTACTCATTGCAACGTTCCCAATTCTCTTCTC
TTTTTTTTTGTCTGGCACATATACAATCTGTATCCGTTTTCAGAATTAATCGAATATTTC
AATATTAATTTTGATCATAAGGATTCTATCAGATAAATTGTAATATTCATCTAATGATGA
CTATTCACCAAATTTACATTCTGCATGATAGCAAAGAGCACTATCTAATCATTAATTTAC
TAGAAAACATATCTTGAAACTTATATATATGATTATCATTCAAACTTATTGCAGTGTTAG
TATTTAGGTTTACATATATTATACATCTCAGAAAGTCTGGCATCTATATATAGTCGATCG
GTGATATGCATGATCAGTTGGGAATAAGCTTGAAAGGAGGTCTCACGAGCTTTGGTTTAG
GACTGGAGTGGCCATA
   
Sequence Name 68-K029747-022-054-C08C-FM19
GenBank Accession LN511927 [GenBank]
Sequencing primer gene specific primer, FM19: 5'-GAATTCAAGAGACTATGGCCACTC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K029747-022-054-C08C-FM19
ACTCCTTTCAGCTTATTCCCAACTGATCATGCATATCACCGATCGACTATATATAGATGC
CAGACTTTCTGAGATGTATAATATATGTAAACCTAAATACTAACACTGCAATAAGTTTGA
ATGATAATCATATATATAAGTTTCAAGATATGTTTTCTAGTAAATTAATGATTAGATAGT
GCTCTTTGCTATCATGCAGAATGTAAATTTGGTGAATAGTCATCATTAGATGAATATTAC
AATTTATCTGATAGAATCCTTATGATCAAAATTAATATTGAAATATTCGATTAATTCTGA
AAACGGATACAGATTGTATATGTGCCAGACAAAAAAAAAGAGAAGAGAATTGGGAACGTT
GCAATGAGTAAATGAGTATGATGGTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGAT
AAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAA
TTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11