Sequences from confirmation amplicon


Confirmation sequences for line 054D03 and hit At1g49890 are shown.
Line ID 054D03
Locus At1g49890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18471934
Primers used for PCR 07cy - 8474, show primer details
   
Sequence Name 29-K109134-0022-054-D03C-07cy
GenBank Accession LN499325 [GenBank]
Sequencing primer gene specific primer, 07cy: 5'-AAGTGTTAAGTTGTGGGATGGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K109134-0022-054-D03C-07cy
ACTTCAGAGGAGATATAGAAAATCTACCTACCATACATAAATAAATTATTAGAGTCCAAA
TTCATTTGCTAAACACGTTGCGAGACAATTACCATTCAACAATAATTGAGTTATACACAA
AACTTGATTTTCTTATTACGGCAAGAAGGAATCACTAAGCCAAATTCCAAAGAATATCTG
TCGTATTCCTCAAAAATAGAAGGAGAAGAAAACGTCTTAATTAGGACATTAGCCCTTTTA
AAGTAAGAAATCGCATAAACTTAAGAGAGTTCATTCACAGTCTTACCTGTCCCCTCAGAA
TAGAAGCCAGTTTCAATTTCTGCCTTAGCAAAAGCAACTTGATTCGTTTTAAAGTGACAC
TGTGCCGCAGTTCCGAGATTGATACCCATGCATTCCACAGGTTTTTCTGGCAAAGAGAAA
AAACAAGAGAGTCAAATTACAGTTTATGTTACGCCGCTNATAGTATAGATTTAGTCATCC
TTAAACAAAAACTACTCTTCCACTATATTAGTTCCAGTAAGATTAACAAACTCATGCTTA
ATCTCCAAATTGACCTGATAAATACAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 13-K109133-0022-054-D03C-8409
GenBank Accession LN492077 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K109133-0022-054-D03C-8409
CATTTACAATTGTATTTATCAGGTCAATTTGGAGATTAAGCATGAGTTTGTTAATCTTAC
TGGAACTAATATAGTGGAAGAGTAGTTTTTGTTTAAGGATGACTAAATCTATACTATTAG
CGGCGTAACATAAACTGTAATTTGACTCTCTTGTTTTTTCTCTTTGCCAGAAAAACCTGT
GGAATGCATGGGTATCAATCTCGGAACTGCGGCACAGTGTCACTTTAAAACGAATCAAGT
TGCTTTTGCTAAGGCAGAAATTGAAACTGGCTTCTATTCTGAGGGGACAGGTAAGACTGT
GAATGAACTCTCTTAAGTTTATGCGATTTCTTACTTTAAAAGGGCTAATGTCCTAATTAA
GACGTTTTCTTCTCCTTCTATTTTTGAGGAATACGACAGATATTCTTTGGAATTTGGCTT
AGTGATTCCTTCTTGCCGTAATAAGAAAATCAAGTTTTGTGTATAACTCAATTATTGTTG
AATGGTAATTGTCTCGCAACGTGTTTAGCAAATGAATTTGGACTCTAATAATTTATTTAT
GTATGGTAGGTAGATTTTCTATATCTACCTCTAGAAGTTGGTATGCTTACTTGCTTAGAA
CCATCCCACAACTTAACAC

Last Updated on 06.12.2018 16:11