Sequences from confirmation amplicon


Confirmation sequences for line 054D03 and hit At4g35830 are shown.
Line ID 054D03
Locus At4g35830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16978282
Primers used for PCR 22vn - 3144, show primer details
   
Sequence Name 05-K117806-0022-054-D03C-22vn
GenBank Accession LN488537 [GenBank]
Sequencing primer gene specific primer, 22vn: 5'-AAAAATAGCTGGAAAATCAGCATC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K117806-0022-054-D03C-22vn
TTTTGGATCGTAACAGATAAATCGATACCCATGGATGATAGATACAAGTCGAGTGAAGAA
GCAAAGTAGAGAGATCTGTTTTCAGAAGAGAGAAAGTTTATAAGCGGAGTTGGTGAGGCT
ACACGAGCCTGAGCCGATTCAGATTTTTTTTATATGTAAGAAGGAAATAAATCTGACAGA
TCCGTGGTGGGCTCTTTTCGTCAGAACTGATGGCTGTTTGATTTTTATTTTTATTTTATA
TTCTATTTTCTTAATTATCCAACTTTTTTTTTTGGGGAATACCCTTTCTTGGCTTTTTTT
TGTACCAGGGGAATTATATCTGTTCAACCAGGATTTTTTTTTCACAAACTTATAACCCCC
CAATCGAATAGGGGCCCCTCCCCTATTCCCTTTAAACCAAATTCATTTAAAACTGGCGGA
TCTTTTACAATGGAAAAGGCGAATATTTAAACCCAAAGGGGGGAAACAACAATCTGATCC
CGGGGTACCCGGCCCTCTCCAAAGGAAAGGAATTTCCTTATATAAAGAAGGGGTCTTGCA
AAGGAAAGGGGAATTGTGCGTCACCCCTTAAATCAGTGAAAA
   
Sequence Name 05-K117784-0022-054-D03C-3144
GenBank Accession LN488536 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K117784-0022-054-D03C-3144
ATCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGA
CAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGCATTTAC
AATTGTAAATGATCAGCATGTCTTAATTGAATATGATTCAACGGATATAGAGAAGAGGCA
CATATCAGATTGGTGGATTATAAGTTTGTGAAAATAAAATCATGATTGAACAGATATAAT
TCACTTGATACAAAAAAAAGCAAAGAATGGATATTCACAAAAAAAAAAATTTGGATATTT
AAAAAAATAGAATATAAAATAAAAATAAAATTCAAACGCCCTTCATTTCTGACAAAAAAA
CCCCCCCACGGATCTGTCAAATTTATTTCCTTCTTACATATAAAAAAAATCTAAATCGGC
TCAGGCTCGGGTACCCCCACCAACCCCCCTAATAAACTTTCTCTCTTCTAAAAACAAATT
TCTCTACTTGGCTTCTTCACTCAACTTGTATCTTTCACCCAGGGGTATCGATTTATCTTT
TTACAATCCAAAAGGATCCAGGTTTCTAATTTCTCCTTCCCCCCAAAACTAATTT

Last Updated on 06.12.2018 16:11