Sequences from confirmation amplicon


Confirmation sequences for line 054G06 and hit At3g58580 are shown.
Line ID 054G06
Locus At3g58580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21663587
Primers used for PCR 09xy - 3144, show primer details
   
Sequence Name 26-K119596-0022-054-G06C-09xy
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 09xy: 5'-GTCAAAATGGTACTAGGATGTCCG-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K119596-0022-054-G06C-09xy
ACACCTCGAACTTCAAAACGTGGCTAATATCATCAGCTGTTGGAGTGTACGTTTTGCAAC
CCCCAACCTCAACTAAAGTCTCTCCTCCATTTTTCTGAGTAATATTAGAAGGATAAAACG
GAGCTGGACCATTGTTGGCAATACTAAGATTTGACATAGACCCAGATAAGGAACCAGCGA
GAGACCCAGAACCCGCACTGTTATTCCTGTTCAAGTCATCATCCTCGTTTGCGTTGTTTT
CCGCAGCTGCTCGCTCGTGCAAAGTCCTGTGATGCTGCCACGCATCCGTGAAGCATTTTG
GCGAACAGTGATAGCTTTTTGGAACAAGGGATCTACGCTTAGAACAGAAAACACACTGTA
AAGTAGCTTGTTCCGTTGGATGCACACTGCAAATCGTTACTTTCTTATCACTCTGTACAC
GATACCTACAACAAATAAATCCAAAAAATGAATCTACAACAAGATCAGAACTTTACACAC
AAAAACCATTTGACGACCTAAATCAGAACATCTACATGAGAAATCTCTGAATTCAGATCA
AAATTCAATCTTTGATAAACCCCAATTCGCCAAATGATCCAAGAAAGCAATTAGACAAAG
AGCCAATCAGAAATCTAACCATCTGTATCTCAAGAAGTAGCCTTCAAGAGGAGCAGATTC
AGGGACGCTCCGTCGATCACTAGTCCGCCGTCGGCCGGCGCGACCAAACACTGATAGTTT
AAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAAT
GAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTT
ACGTCAGTGG
   
Sequence Name 02-K119595-0022-054-G06C-3144
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K119595-0022-054-G06C-3144
TTCGCAGACCCTTCCTCTATTAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGG
GGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGGTCGCGCCGGCCG
ACGGCGGACTAGTGATCGACGGAGCGTCCCTGAATCTGCTCCTCTTGAAGGCTACTTCTT
GAGATACAGATGGTTAGATTTCTGATTGGCTCTTTGTCTAATTGCTTTCTTGGATCATTT
GGCGAATTGGGGTTTATCAAAGATTGAATTTTGATCTGAATTCAGAGATTTCTCATGTAG
ATGTTCTGATTTAGGTCGTCAAATGGTTTTTGTGTGTAAAGTTCTGATCTTGTTGTAGAT
TCATTTTTTGGATTTATTTGTTGTAGGTATCGTGTACAGAGTGATAAGAAAGTAACGATT
TGCAGTGTGCATCCAACGGAACAAGCTACTTTACAGTGTGTTTTCTGTTCTAAGCGTAGA
TCCCTTGTTCCAAAAAGCTATCACTGTTCGCCAAAATGCTTCACGGATGCGTGGCAGCAT
CACAGGACTTTGCACGAGCGAGCAGCTGCGGAAAACAACGCANACGAGGATGATGACTTG
AACAGGAATAACAGTGCGGGTTCTGGGTCTCTCGCTGGTTCCTTATCTGGGTCTATGTCA
AATCTTAGTATTGCCAACAATGGTCCAGCTCCGTTTTATCCTTCTAATATTACTCAGAAA
AATGGAGGAGAGACTTTAGTTGAGGTTGGGGGTTGCAAAACGTACACTCCAACAGCTGAT
GATATTAGCCACGTTTTGAAGTTCGAGTGTGTTGTGGCTAATGCTGAGACTAAACAGATT
GTCGGACATCCTAGT

Last Updated on 06.12.2018 16:11