Sequences from confirmation amplicon


Confirmation sequences for line 054G07 and hit At1g12070 are shown.
Line ID 054G07
Locus At1g12070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4079805
Primers used for PCR EL01 - 3144, show primer details
   
Sequence Name 01-K025673-022-054-G07C-3144
GenBank Accession LN486425 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K025673-022-054-G07C-3144
AGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCA
TTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGGTTTCCCGCCTTCCATCT
CCTCAAGCTTTCATCATCCTAGACAAATTTTTAATTGTAGAAAAGCGCTATAGCCAAAAT
GCAATTGGGTTTGAATAATTTATGAAATCATAACAAGAGAGATCAAAACCTTGTCTTTCT
CGAGCTGTTCTTTAAGAGCAATCATGGGACCAAGTTCGAGTTTCTTGTCTTCTTCTTCTT
CATCATCATCTGTCGGACACATAGAGCTATGGCTATTTTTCCTGCTTAGCCCTTGTCCCT
TTGGCTCTTCTGAAGACTCTCCAGCTTTCATCTCCTCATCGTTCAAAACCATCTTTGCTT
TCCTGATCTCTATTCGTTCTCTGATTTAATTCAGATTTGTCGTAGAAAATCTCTACCGTT
GGATTTGATTC
   
Sequence Name 02-K025673-022-054-G07C-EL01
GenBank Accession LN486881 [GenBank]
Sequencing primer gene specific primer, EL01: 5'-TAAATCATGTCGAATCAAATCCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K025673-022-054-G07C-EL01
ATTCCCCGGAAATTTTCTACGACTAATCTGAGTTAAATCAGAGAACGAATAGAGATCAGG
AAAGCAAAGATGGTTTTGAACGATGAGGAGATGAAAGCTGGAGAGTCTTCAGAAGAGCCA
AAGGGACAAGGGCTAAGCAGGAAAAATAGCCATAGCTCTATGTGTCCGACAGATGATGAT
GAAGAAGAAGAAGACAAGAAACTCGAACTTGGTCCCATGATTGCTCTTAAAGAACAGCTC
GAGAAAGACAAGGTTTTGATCTCTCTTGTTATGATTTCATAAATTATTCAAACCCAATTG
CATTTTGGCTATAGCGCTTTTCTACAATTAAAAATTTGTCTAGGATGATGAAAGCTTGAG
GAGATGGAAGGCGGGAAACCGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAA
ATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCC
TTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGGTGGAAACGTCT
CTTTTTCACGATGCTCCT
Primers used for PCR FI21 - 3144, show primer details
   
Sequence Name 84-K118835-0022-054-G07C-FI21
GenBank Accession LN514480 [GenBank]
Sequencing primer gene specific primer, FI21: 5'-TAGAGCCTTCTTTGAGAGTGAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K118835-0022-054-G07C-FI21
TTTCGTTTCGGGATCGTTATACCATGTCTTCTCTATCCGGTGACCTAATTGTTAAGGTTA
GTATCTTTACCAGTGGATCCGGAGTCTCTGCATTTACATTTTGTCACTCTATCAAAACAG
TACATATATATATATATATATATATATATATATTTTTTAATATTCCCACAATTATCTTAT
CTATAGAAATCTAAAAAGTAATTCTAATCAGATTCATCTGTTATTTAACCATATATATAA
ACCAGATATTGGATTTTGATTTTAGATACATCTGTTATTTTTTCGGTTTAAACTATCAGT
GTCTCCCAGAGATGGAAGGCCACACACTGATATTTTAAACCGAAGGCGGGAAACGACAAT
CTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGG
TCTTGCGAAAGAGAGTGAGATTGTGCGTCATCCCTTACGTCTGTAGAGATAACACATCAC
TCCACA
   
Sequence Name 60-K118834-0022-054-G07C-35St
GenBank Accession LN510380 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K118834-0022-054-G07C-35St
CTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCG
GGGATCAGATTGTCGTTTCCCGCCTTCGGTTAAACTATCAGTGTTTGGCCTTCCATCTCC
GGGAAACACTGATAGTTTAAACCGAAAAAATAACAGATGAATCCAAAATCAAAATCCAAT
ATCCGGTTTATATCTATGGTTAAATAACAGATGAATCCGATTAGAATTACTTTTTAGATT
TCAATAGATAAGATAATTGTAGGAATATTAAAAAATATATATATATATATATATATATAT
ATATGTACTGTTTTGATAGAGAGACAAAATGTAAATGCAGACACTCCAGATCCACTGGTA
AAGATACTAACCTTAACAATTATGTCACCAGATAGAGAACACATGGTATTAACGATCCCT
GAAAACAGAAAACCGGCTTCAAAAGGACCATGGTTCACTCTCAAAAAAAGCTCAAAA

Last Updated on 06.12.2018 16:11