Sequences from confirmation amplicon


Confirmation sequences for line 054G08 and hit At3g25840 are shown.
Line ID 054G08
Locus At3g25840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9456644
Primers used for PCR DE12 - 8474, show primer details
   
Sequence Name 09-K019240-022-054-G08C-8409
GenBank Accession LN489973 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K019240-022-054-G08C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTTCAGAGAGAGTTAGTAA
ATTAAGTTATGATAATGGCAGAAGTTCGTTTTCTCCGTCAAACAGCAGGCAGTCTAACGA
AGGCCGTGCCCGGAGTAGATCTAAGTCACATGATCGGGAGAGAGAAACTTCTAGGTCAAG
GTTTGCGGAGGATGAGTTTTCGGACAGAGGAAGACATCATGATTCTAGTAGGGATTATCA
TCATGACAGAGTTGACTCAGTTAGGACTGAGGAAAGATATCATCGACGTGGGCGATATGA
GGAAAATGACAGGCAATATAGTCGAGAAGTCTTGGAAAGAGATAGAAGTAAGGAGAGAGA
TATGGACAGGGAGGGAAGCATAAGAGATAGGGATTCGGAAGGAAGTAAGCGGAGAGATAN
AGATAGTGAT
   
Sequence Name 10-K019240-022-054-G08C-DE12
GenBank Accession LN490444 [GenBank]
Sequencing primer gene specific primer, DE12: 5'-TCACCGTAGTCCACATCACTACTT-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K019240-022-054-G08C-DE12
ACTTCTCCCTATCTTTCTGGGTTTCTCTTCCTCTCTCCCAATCTTTTTCACCCTCCCTTT
CCTTTTCTCTCTTCCTCTCTCTGCTGTTATACCTGGTTCTATCTCTACTCCTACCTCTCT
CATTGGCTCTATCCCGTAAATAATCTCCTTCCCTCTCCTTTCTCCTATCCCTATCAATGC
TGCGCTCTCGTTCCTTCTCTTTTCGTCTTTCACGGTCAGACTCTATCTCCCTTCTCTTCT
CTCGTTCTCTTTCCCTCCTCCGATCACTATCTTTATCTCTCCGCTTACTTCCTTCCGAAT
CCCTATCTCTTATGCTTCCCTCCCTGTCCATATCTCTCTCCTTACTTCTATCTCTTTCCA
AGACTTCTCGACTATATTGCCTGTCATTTTCCTCATATCGCCCACGTCGATGATATCTTT
CCTCAGTCCTAACTGAGTCAACTCTGTCATGATGATAATCCCTACTAGAATCATGATGTC
TTCCTCTGTCCGAAAACTCATCCTCCGCAAACCTTGACCTAGAAGTTTCTCTCTCCCGAT
CATGTGACTTAGATCTACTCCGGGCACGGCCTTCGTTAGACTGCCTGCTGTTTGACGGAG
AAAACGAACTTCTGCCATTATCATAACTTAATTTACTAACTCTCTCTGAAGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA

Last Updated on 06.12.2018 16:11