Sequences from confirmation amplicon


Confirmation sequences for line 054H02 and hit At1g72260 are shown.
Line ID 054H02
Locus At1g72260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27198909
Primers used for PCR 01kt - 3144, show primer details
   
Sequence Name 36-K113817-0022-054-H02C-01kt
GenBank Accession LN502301 [GenBank]
Sequencing primer gene specific primer, 01kt: 5'-ACCATTCAAAAGATGAAAGGAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K113817-0022-054-H02C-01kt
CACAAGTTCAGTAGAAGCAAAGATCTGCTGCCCTTCCACCAAGCTAGAAATGGCTATAGT
GTATGCCGTATAAGGTTTTCCAAGGGAAGGTGTATGCAAGTGAGTGGATGCCAAAACTCT
GATACATGCCCTCGAGGTTGGGTAAACGCCATTCTCGAAAACTCAGGTAAATAGGAAAAT
GTCTTTGATGTTATTTGTTAAAAGATCGATCAAGATTTTAATACTTTAAAAAAAAACTCA
GCTGATGCTACCAATGAGCACTGCAAGTTAGGGTGTGAAACTTCTGTGTGTGGTGCCATG
AACACTCTCCAGAACTCTGGTAAAGCGAACTCCTTAAGTCTCATTTTTATCATTATTTTG
TTAGAATATATTCTAATGTTTGTTTCGGTGACTAGAAAATCTGAATCCTTATATAGAGGA
AGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATAT
   
Sequence Name 10-K113816-0022-054-H02C-3144
GenBank Accession LN490741 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K113816-0022-054-H02C-3144
CCTTCGCAGACCCTTCCTCTATATAGGATTCAGATTTTCTAGTCACCGAAACAAACATTA
GAATATATTCTAACAAAATAATGATAAAAATGAGACTTAAGGAGTTCGCTTTACCAGAGT
TCTGGAGAGTGTTCATGGCACCACACACAGAAGTTTCACACCCTAACTTGCAGTGCTCAT
TGGTAGCATCAGCTGAGTTTTTTTTTAAAGTATTAAAATCTTGATCGATCTTTTAACAAA
TAACATCAAAGACATTTTCCTATTTACCTGAGTTTTCGAGAATGGCGTTTACCCAACCTC
GAGGGCATGTATCAGAGTTTTGGCATCCACTCACTTGCATACACCTTCCCTTGGAAAACC
TTATACGGCATACACTATAGCCATTTCTAGCTTGGTTGGAAGGGCAGCAGATCTTTGCTT
CTACTTGAACTTGTGCCATGACCAGACTCATTATGAGCAAACTCAAAATCAAAATTCTTC
CTTTCAC

Last Updated on 06.12.2018 16:11