Sequences from confirmation amplicon


Confirmation sequences for line 054H03 and hit At2g25340 are shown.
Line ID 054H03
Locus At2g25340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10793199
Primers used for PCR CN04 - 8474, show primer details
   
Sequence Name 13-K027486-022-054-H03C-8409
GenBank Accession LN491886 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K027486-022-054-H03C-8409
GCCATTTACATTGAATATATCCTGAATTGCGCGGCCATAAGTCCGAACAAACCTCTGATG
AATATCCTCTAGAAACGAAAATGGAATCCTCCCTGAAAATAACACTCAAAACAAGTACAT
TAACACATAACAACAAACTAAAGAAAGAAAGAAAAAAGAATCTCAATCTCTACTACTTCA
CAACTTTGGGAACATTTTAAAGACTCTTCTTATTTCATTGTCTCTGTTCTCAAAGGTTGT
TACTCATCATAAACACCAAATTCATAAAACCTCAAATTTCAAGAATCTGGTCTCTGTCTT
CTAAACTAAGTAATGATTAATTTATTGATCATTAACCAAAATAAGGAACAAAATATTGAG
TATTAGTGTTATCATAGTAACAGACTAAAAGCTCAAAAGACTTACTTCCGGCGTCTTCAT
CAGCCATACAAAGAACGGTGAGGCCATCAGTAC
   
Sequence Name 14-K027486-022-054-H03C-CN04
GenBank Accession LN492357 [GenBank]
Sequencing primer gene specific primer, CN04: 5'-TCTTCCACGTAAAACGTACTGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K027486-022-054-H03C-CN04
GCCGGAAGTAGTCTTTTGAGCTTTTAGTCTGTTACTATGATAACACTAATACTCAATATT
TTGTTCCTTATTTTGGTTAATGATCAATAAATTAATCATTACTTAGTTTAGAAGACAGAG
ACCAGATTCTTGAAATTTGAGGTTTTATGAATTTGGTGTTTATGATGAGTAACAACCTTT
GAGAACAGAGACAATGAAATAAGAAGAGTCTTTAAAATGTTCCCAAAGTTGTGAAGTAGT
AGAGATTGAGATTCTTTTTTCTTTCTTTCTTTAGTTTGTTGTTATGTGTTAATGTACTTG
TTTTGAGTGTTATTTTCAGGGAGGATTCCATTTTCGTTTCTAGAGGATATTCATCAGAGG
TTTGTTCGGACTTATGGCCGCGCAATTCAGGATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAA
CAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA
Primers used for PCR CN03 - 8474, show primer details
   
Sequence Name 21-K027487-022-054-H03C-8409
GenBank Accession LN495521 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K027487-022-054-H03C-8409
CCATTTACTCAAGAGTTCTTAACCAACAAATTGAGTATTACTCTAATGATCCTAATGCCG
ATACGATAAGTCGAATTAAGGGTGAAATGAATCAGGTTTTGTGTTTCTCCATAATTTGAA
ACATATATAGTGCAATGGTTATGTTTTGCTGGCTTCAACGGATTCTCTGTTTTGTATGGT
TATGAATAGGTGAGGGATGTTATGATAGAGAACATTGACAACATTCTTGATAGAGGTGAA
CGTCTTGAGCTTCTTGTCGATAAAACCGCGAACATGCAAGGGAATACCTTCCGATTCAGA
AAGCAAACACGTCGTTTCAACAACACGGTGTGGTGGAGAAATTGCAAGCTCACGTATGTT
TTCATCAACCGTAATTCCTTACCATGTTTGCACCTTAAGCATGACTTCAAATGGTTTAAT
CTTTTCGATGCATTCTCGTGTAACGTCTCTTGTAGGCTACTCTTAATACTAGTGTTGTTG
GTGATCATATACATCGGTGTGGCCTTTGCTTGCCATGGACCAACTCTACCCTCTTGCGTT
   
Sequence Name 22-K027487-022-054-H03C-CN03
GenBank Accession LN495968 [GenBank]
Sequencing primer gene specific primer, CN03: 5'-CTCACTTAATTAAACGCAAGAGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K027487-022-054-H03C-CN03
CCGATGTATATGATCACCAACAACACTAGTATTAAGAGTAGCCTACAAGAGACGTTACAC
GAGAATGCATCGAAAAGATTAAACCATTTGAAGTCATGCTTAAGGTGCAAACATGGTAAG
GAATTACGGTTGATGAAAACATACGTGAGCTTGCAATTTCTCCACCACACCGTGTTGTTG
AAACGACGTGTTTGCTTTCTGAATCGGAAGGTATTCCCTTGCATGTTCGCGGTTTTATCG
ACAAGAAGCTCAAGACGTTCACCTCTATCAAGAATGTTGTCAATGTTCTCTATCATAACA
TCCCTCACCTATTCATAACCATACAAAACAGAGAATCCGTTGAAGCCAGCAAAACATAAC
CATTGCACTATATATGTTTCAAATTATGGAGAAACACAAAACCTGATTCATTTCACCCTT
AATTCGACTTATCGTATCGGCATTAGGATCATTAGAGTAATACTCAATTTGTTGGTTAAG
AACTCTTGAGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCG
TCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGAC
GTGTCTA

Last Updated on 06.12.2018 16:11