Sequences from confirmation amplicon


Confirmation sequences for line 054H11 and hit At3g51520 are shown.
Line ID 054H11
Locus At3g51520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19110453
Primers used for PCR MP31 - 8474, show primer details
   
Sequence Name 51-K021738-022-054-H11C-8409
GenBank Accession LN507367 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K021738-022-054-H11C-8409
CCATTTACAATTGAATGTCCTTTGGCCCATATTAGCTTTAACAAAGGCTTAACNCTTTTG
AAAACTCCAGTTAGGCAATAATTATTACGGGTCAAATGACAAAGAATGAACCTTGTCGGG
CTCTTTAAAGATGGGTTGTTGTATAAAAAGTTGGGTAGTTAAAAGATTGGTTATTTGGGC
TCTGCACTCAAGTGAGAGAGAAGATAGATAGATCTGAGTAGAATCTTCGATTCATTATTC
GTTGTCGTCGTTCATCTGTGAGAAGCGGACAAACCAAAGAATCCACCGGAGCTAGTGATA
TGGGTGGTTCCAGAGAGTTCCGAGCTGAGGAACATTCAAATCAATTCCACTCTATCATCG
CCATGGCCATCTGGCTTGGCGCCATTCACTTCAACGTCGCTCTTGTTCTCTGTTCTCTCA
TTTTCCTTCCTCCTTCTCTATCTCTCATGTATATACATCTCTCTCTCTATCTCTCTCTGT
TTGCTGTTACAAGTCTCAAATTTAAACTCTTTGAAATCCTCTGTTTTTCTCAGGGTCTTG
GGCTTGCTCTCTCTGTTTATCTTTATCCCAATCGATCATCGTAGCAAATATGGTCGTAAG
CTCGCTAGGTACATATGCAAGCACGCGTGTAATTATTTCCCCGTCTCTCTGTACGTCGAG
GATTACGAAGCTTTCCAGCCTAATCGTGCCTAT
   
Sequence Name 52-K021738-022-054-H11C-MP31
GenBank Accession LN507683 [GenBank]
Sequencing primer gene specific primer, MP31: 5'-AGGAGATTGTGACTAACCATAGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K021738-022-054-H11C-mp31
GACGTCAGAGAGACGGGGAAATAATTACACGCGTGCTTGCATATGTACCTAGCGAGCTTA
CGACCATATTTGCTACGATGATCGATTGGGATAAAGATAAACAGAGAGAGCAAGCCCAAG
ACCCTGAGAAAAACAGAGGATTTCAAAGAGTTTAAATTTGAGACTTGTAACAGCAAACAG
AGAGAGATAGAGAGAGAGATGTATATACATGAGAGATAGAGAAGGAGGAAGGAAAATGAG
AGAACAGAGAACAAGAGCGACGTTGAAGTGAATGGCGCCAAGCCAGATGGCCATGGCGAT
GATAGAGTGGAATTGATTTGAATGTTCCTCAGCTCGGAACTCTCTGGAACCACCCATATC
ACTAGCTCCGGTGGATTCTTTGGTTTGTCCGCTTCTCACAGATGAACGACGACAACGAAT
AATGAATCGAAGATTCTACTCAGATCTATCTATCTTCTCTCTCACTTGAGTGCAGAGCCC
AAATAACCAATCTTTTAACTACCCAACTTTTTATACAACAACCCATCTTTAAAGAGCCCG
ACAAGGTTCATTCTTTGTCATTTGACCCGTAATAATTATTGCCTAACTGGAGTTTTCAAA
AGGTTAAGCCTTTGTTAAAGCTAATATTGGGCCAAAGGACATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAA
ATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCA
ATAAACAAATTATTCTAATTCGGAAATCTNTATNTCGACGTGTCTA

Last Updated on 06.12.2018 16:11