Sequences from confirmation amplicon


Confirmation sequences for line 055A05 and hit At2g24645 are shown.
Line ID 055A05
Locus At2g24645 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10481103
Primers used for PCR 04sm - 8474, show primer details
   
Sequence Name 36-K116453-0022-055-A05C-04sm
GenBank Accession LN502363 [GenBank]
Sequencing primer gene specific primer, 04sm: 5'-GTTGGTATCTTTATTTTGTGCGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K116453-0022-055-A05C-04sm
GTTGAACTCTCCTTTTCTTTGCTAAACAAACATTCACAAATCCCACACCGTAAGGCCAAT
GATCATACAATTACAACATACTTAGAAAACATACTACAATAGTACAATTAGACCTTAAGA
GTTATATCTTACCAAATAAAAGAGTCAAACATTGATTCATTTCAATATTACACTCATATA
CATTTTCATTTGATTGCTAGCTTCACCTTGGAGCAGAACTTAAGGACGCAACTTGTTTCT
TCTTCCCAAATCAGTTCCAACATGATGGATTCGTTTGCCTTCACACCGTTAGCTTGGATG
AATCCTTTCCAGCCTCCTACCATTCCCAGTCTTTTTCTTTTGTAGTCCACCAACCGCAGA
GTCGTTAACCACTTCATACCATGTTTATCCAACAGAGTCATTTTAGTTTCTTCATTGATA
CCATTCATCCTCGTGAAGGGTATCGGAAGACGCTGAAACAAAGATTCACACAAAAGATTC
AAATTTGATGGACTGTAAAAAGACAATGGCTTGGAGTTTTGAAACTAACCAGTGCAGAAC
GTAGGATGTTATAAGGTGTAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGA
   
Sequence Name 03-K116452-0022-055-A05C-8409
GenBank Accession LN487641 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K116452-0022-055-A05C-8409
ATTGATATTACACCTTATAACATCCTACGTTCTGCACTGGTTAGTTTCAAAACTCCAAGC
CATTGTCTTTTTACAGTCCATCAAATTTGAATCTTTTGTGTGAATCTTTGTTTCAGCGTC
TTCCGATACCCTTCACGAGGATGAATGGTATCAATGAAGAAACTAAAATGACTCTGTTGG
ATAAACATGGTATGAAGTGGTTAACGACTCTGCGGTTGGTGGACTACAAAAGAAAAAGAC
TGGGAATGGTAGGAGGCTGGAAAGGATTCATCCAAGCTAACGGTGTGAAGGCAAACGAAT
CCATCATGTTGGAACTGATTTGGGAAGAAGAAACAAGTTGCGTCCTTAAGTTCTGCTCCA
AGGTGAAGCTAGCAATCAAATGAAAATGTATATGAGTGTAATATTGAAATGAATCAATGT
TTGACTCTTTTATTTGGTAAGATATAACTCTTAAGGTCTAATTGTACTATTGTAGTATGT
TTTCTAAGTATGTTGTAATTGTATGATCATTGGCCTTACGGTGTGGGATTTGTGAATGTT
TGTTTAGCAAAGAAAAGGAGAGTTCAACTTTATCAGTTTAAATTAGTGACTAAAACATTT
TGTGAAGATTTTTACCGCACAAAATAA

Last Updated on 06.12.2018 16:11