Sequences from confirmation amplicon


Confirmation sequences for line 055B03 and hit At5g52605 are shown.
Line ID 055B03
Locus At5g52605 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21345542
Primers used for PCR 05xr - 8474, show primer details
   
Sequence Name 39-K119512-0022-055-B03C-05xr
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 05xr: 5'-TATAAAAGTTCGTCATAGAGCGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K119512-0022-055-B03C-05xr
ACGATCTAGTTTTGGTTAGAGTTCAACAAGAATATTACGACAACGGATATGTAGAAGATG
TATGTTTGATGTATTTCACAAAGAGATACAGAGCTTGTTTATATACAAGTAATAGGCGTG
AGCCTCAAGATTATATTAATTTCCTAAACTAGTGAATTGGAATATTGAGAAATGAATGAG
TCTCTAATCATATCTCTTGACAGAATAAACCCACAGAAAATATTAGTCTGCATTACCTTG
CAAACTACCAAAAACGTTTATCTAAATAAGTCATGTATGTCTTGTAATAATGTAGTCAAA
GTTGGAATATCCAATCTTTTTGGAAACATGTTTTTAATTTGGTTGACCAAATATATTGTA
TTCCTTACAAATTTATTATGTGAGATAATGTTAATTCGAAGGATCCCTTGAATATAGTTA
GAATTCTAATTATAAATACGAAACAACTCCTTCTTCATTCTCATTCAAATACAAAAATAT
CAAAAAATTCTTAATATTTTGATTTAACAAAGAAAAAAGAGAAGAATGGTTAGAACAAAT
GTTGTCTCTTTTGTTTTATTTGCTGCTATTGTATTATGTATACTCATCATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 07-K119511-0022-055-B03C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K119511-0022-055-B03C-8409
CATTTACATTGAATATGATGAGTATACATAATACAATAGCAGCAAATAAAACAAAAGAGA
CAACATTTGTTCTAACCATTCTTCTCTTTTTTCTTTGTTAAATCAAAATATTAAGAATTT
TTTGATATTTTTGTATTTGAATGAGAATGAAGAAGGAGTTGTTTCGTATTTATAATTAGA
ATTCTAACTATATTCAAGGGATCCTTCGAATTAACATTATCTCACATAATAAATTTGTAA
GGAATACAATATATTTGGTCAACCAAATTAAAAACATGTTTCCAAAAAGATTGGATATTC
CAACTTTGACTACATTATTACAAGACATACATGACTTATTTAGATAAACGTTTTTGGTAG
TTTGCAAGGTAATGCAGACTAATATTTTCTGTGGGTTTATTCTGTCAAGAGATATGATTA
GAGACTCATTCATTTCTCAATATTCCAATTCACTAGTTTAGGAAATTAATATAATCTTGA
GGCTCACGCCTATTACTTGTATATAAACAAGCTCTGTATCTCTTTGTGAAATACATCAAA
CATACATCTTCTACATATCCGTTGTCGTAATATTCTTGTTTGAACTCTAAACCAAAACTT
AGATCGGTTTGGTTTACTCGCCGGTTGTTGCGCTCTATGACAAANTTTTTAT

Last Updated on 06.12.2018 16:11