Sequences from confirmation amplicon


Confirmation sequences for line 055B11 and hit At4g26160 are shown.
Line ID 055B11
Locus At4g26160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13255055
Primers used for PCR PG03 - 8474, show primer details
   
Sequence Name 14-K016593-022-055-B11C-8409
GenBank Accession LN492281 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K016593-022-055-B11C-8409
CATGTAGATTTCTCCGACGACGACATCGTTACGATCCCTAAGCTTCTCGCTTTACGCTTC
TTCTAATTCTACTCCGATTTCTACACCAATTGAGGCCCGACAGTTACTGAGTTCATGCAA
TAGATTCTACGGTCTGTCCTCTTCGAGCTCTTCTTCCTCCCTTACTACTTCATCTTTGAT
CGGAAATCTTGTTTTCTCATCAAGGAATCAGTCTCTCTCCGTAAAGGTATACAATTTCGT
AAATTTTTGTGATGGATTTCAACTTTACGGCCTAAATTCGAGAACCCATGTTCCACTTAT
TGAGAAATCTGCTTAATTCTTATGTCACTTGTAGTCGATTGGGAAATTAGGGATTTCTAT
ATGTGCCATTGATTGTGATTACTCTTTATGTGGTCTTTTTGGTCCATTTGAAGATGTTTT
AAGTTAGGTCTCTCAGCCACATAGACGATTACATTAGTCCTGTGTTTAAGTTCACACTGT
CTTACTTTGATTGAAGCTAGGTTGCGTTAAGGGCTTTTAAATAACATCTAACTTTATGAG
TGTTTGGTGTTGTCAGTGTGGCTACATATTGATGAAATGTATGTGTGTTTTGGGATAGGT
CCAAGCGTTAGCTGCTGAAACCGAACAGCCAAAATGGTGGGAGAGGAAAGCAGGGCCAAA
CATGATTG
   
Sequence Name 18-K016593-022-055-B11C-PG03
GenBank Accession LN494095 [GenBank]
Sequencing primer gene specific primer, PG03: 5'-CAGCTGAGGTAATGTCAATCATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K016593-022-055-B11C-pg03
GCCCTGCTTTCCTCTCCCACCATTTTGGCTGTTCGGTTTCAGCAGCTAACGCTTGGACCT
ATCCCAAAACACACATACATTTCATCAATATGTAGCCACACTGACAACACCAAACACTCA
TAAAGTTAGATGTTATTTAAAAGCCCTTAACGCAACCTAGCTTCAATCAAAGTAAGACAG
TGTGAACTTAAACACAGGACTAATGTAATCGTCTATGTGGCTGAGAGACCTAACTTAAAA
CATCTTCAAATGGACCAAAAAGACCACATAAAGAGTAATCACAATCAATGGCACATATAG
AAATCCCTAATTTCCCAATCGACTACAAGTGACATAAGAATTAAGCAGATTTCTCAATAA
GTGGAACATGGGTTCTCGAATTTAGGCCGTAAAGTTGAAATCCATCACAAAAATTTACGA
AATTGTATACCTTTACGGAGAGAGACTGATTCCTTGATGAGAAAACAAGATTTCCGATCA
AAGATGAAGTAGTAAGGGAGGAAGAAGAGCTCGAAGAGGACAGACCGTAGAATCTATTGC
ATGAACTCAGTAACTGTCGGGCCTCAATTGGTGTAGAAATCGGAGTAGAATTAGAAGAAG
CGTAAAGCGAGAAGCTTAGGGATCGTAACGATGTCGTCGTCGGAGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACG
ACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTA
Primers used for PCR FM27 - 8474, show primer details
   
Sequence Name 02-K035332-022-055-B11C-FM27
GenBank Accession LN486933 [GenBank]
Sequencing primer gene specific primer, FM27: 5'-CAATGTGGTTGTTCTTGTTCATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K035332-022-055-B11C-FM27
TAGTTTGAGAGTTGAATAAGATTACTTTAATTTGTCAAGATCTCGTCAAGTGTTTATACT
TGATGAAAATCTTTTGGGGATCAAAACGGTTTGAAGTAAAGATCGTACTGTCGTACTGGT
GTCTGAAGTAAAGATTGATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAA
ACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTC
GGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGG
   
Sequence Name 02-K035332-022-055-B11C-8409
GenBank Accession LN486932 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K035332-022-055-B11C-8409
GGAATGAAGCATTTACAATTGAATATCAATCTTTACTTCAGACACCAGTACGACAGTACG
ATCTTTACTTCAAACCGTTTTGATCCCCAAAAGATTTTCATCAAGTATAAACACTTGACG
AGATCTTGACAAATTAAAGTAATTCTTATATCAACTCTCAAAACTTAGAAACACAAAAAA
CATGCGAATGAACAAGAACAACCACATTGATCAGCGATGAGTATGAT
Primers used for PCR FL51 - 8474, show primer details
   
Sequence Name 78-K118354-0022-055-B11C-FL51
GenBank Accession LN513744 [GenBank]
Sequencing primer gene specific primer, FL51: 5'-TATCAGCAAGTTTTCCCTCAAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K118354-0022-055-B11C-FL51
CTCTTCGCAGGAATGGTTCACGGGTGATTATTTTAAGTATTTTAATTATAACTCGCATGC
TTAAGAAATAAAATATAAACCATACCATAAGCCCTCATAGCTAGTAATATCTAGATTATT
TGATACATGAAATTTTGTTGCGGTGATATATTAATTTCAGTCAGTCCAACTTTATTATAA
GAGAGTGTTGGGGTTTAGTAGATTTTGTATGAAAGGCTTGTATTTTATTTTTTATGGAAC
TTGATATCCCAACAATGTGGTTGTTCTTGTTCATTCGCATGTTTTTTGTGTTTCTAAGTT
TTGAGAGTTGATATAAGAATTACTTTAATTTGTCAAGATCTCGTCAAGTGTTTATACTTG
ATGAAAATCTTTTGGGGATCAAAACGGTTTGAAGTAAAGATCGTACTGTCGTACTGGTGT
CTGAAGTAAAGATTGATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 67-K118353-0022-055-B11C-8409
GenBank Accession LN511902 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K118353-0022-055-B11C-8409
GCCATTTACATTGAATATCAATCTTTACTTCAGACACCAGTACGACAGTACGATCTTTAC
TTCAAACCNGTTTTGATCCCCAAAAGATTTTCATCAAGTATAAACACTTGACGAGATCTT
GACAAATTAAAGTAATTCTTATATCAACTCTCAAAACTTAGAAACACAAAAAACATGCGA
ATGAACAAGAACAACCACATTGTTGGGATATCAAGTTCCATAAAAAATAAAATACAAGCC
TTTCATACAAAATCTACTAAACCCCAACACTCTCTTATAATAAAGTTGGACTGACTGAAA
TTAATATATCACCGCAACAAAATTTCATGTATCAAATAATCTAGATATTACTAGCTATGA
GGGCTTATGGTATGGTTTATATTTTATTTCTTAAGCATGCGAGTTATAATTAAAATACTT
AAAATAATCACCCGTGAACCATTCCGTGCGATAGAGCCATTAGTAAAATGGCAGCCTCCT
TCTCATCTTGAGGGAAAACTTGGGCTGATA

Last Updated on 06.12.2018 16:11