Sequences from confirmation amplicon


Confirmation sequences for line 055C11 and hit At4g24974 are shown.
Line ID 055C11
Locus At4g24974 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12843216
Primers used for PCR 06rl - 3144, show primer details
   
Sequence Name 70-K115912-0022-055-C11C-06rl
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 06rl: 5'-AGAACAAAGATCTCGGATCACAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K115912-0022-055-C11C-06rl
TTTCCCCCGTCATTTCGAAGGGGAAAACCAATCCAAAGGGTTTCGATATTTTTGTTGAAG
AACAGAGATTTAACATTGTGGTGATCATTGTGTTTGGATTTCTCCATATTGACCATCAAA
CACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTC
TCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGT
GCGTCATCCCTTACGTCAGTGGAGATAT
   
Sequence Name 53-K115911-0022-055-C11C-3144
GenBank Accession LN508259 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K115911-0022-055-C11C-3144
CCCGGGGATTCAGAATTGTCGTTTTCCCGCCTTTCGGTTTAAACTATCAGCGTTTTGATC
GGTCAATATTGGAGAAATCCAAACACAATGATCACCACATGTTAAATCTCTGTCTTCAAC
AAAAATATCAAACCATTTGGATTGGTTTTCCCATTCAAAATGACAAAAAAAAAGAGTCAC
GGTCCAAAAACCAAGAGATGCTCTAAACCCCCAGTGTTGATCAGAGACTAAAGATTGTGA
TCC

Last Updated on 06.12.2018 16:11