Sequences from confirmation amplicon


Confirmation sequences for line 055D01 and hit At1g61520 are shown.
Line ID 055D01
Locus At1g61520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22701271
Primers used for PCR IW61 - 3144, show primer details
   
Sequence Name 08-K033211w-022-055-D01C-IW61
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, IW61: 5'-AGGTACGGCTTAAAAGATAACACG-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K033211w-022-055-D01C-IW61
GTTTTGTCTTCAATCCTTGATAGTGTTTCTCTTTGATAGCACCAACATGGCTTATTGTTA
AATTTGTTTTTGTCTTAAATGTCTAAGGTGGTTTGGGAGATACAGAGTGATAAGATGAGA
CTACTTTTAAAGCATAACAGGTACAGTATTTCATAACATTCTTTGAATATACTAACCGCT
TTGTTTGGTTTGCAAATGAATATACTATCAGTATTTCAAACACTGATAGTTTAAACCGAA
GGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCC
TTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGT
GGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCAC
GATGCTCCTCGTGGGTGGGGGTCCATTCCTTTG
Primers used for PCR FB05 - 8474, show primer details
   
Sequence Name 83-K034733-022-055-D01C-8409
GenBank Accession LN514263 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K034733-022-055-D01C-8409
GCCATTTCAATTGAATATATCCAATTGCAATTGAATACTAACCGCTTTGTTTGGTTTGAG
TATTTCTTACAAAACACGAATATGTTTACACAGGGTGTTACAATACGAAGAAGCCAATGG
TAGCGAAAGATGGAAATATTGTACGATACAAGAGACATAAAAACACATGAGATGATGATC
ACCTCAGTGGAACTTGAGGCTGGTCAAGACGTTGTTGTTGACGGGATCAGCCAAGTGATC
AAGCAGGTTCTGATAAGGTCCCACACCGGTCACTAGTCCTTGGATAAAGTAACCGAGGAT
GGCGAGCATAGCCAGTCTACCGTTCTTGACCTCCTTGAGTTTCAACTCCTTCAGTGACTT
CTCATCTTTCCCAAACCCAAGAGGGTTGAAGAAAGGTCCACCGGGGTAAGCCGGGTTACC
TGAACCGGCCAAACCCTTCTCTAACCCCAAGAAGTACTGTTTTCCCATAGATCCTGGGTT
GTACCAGTCCTGTAACCTCCGGTGCTCAGCGAATCCCATCAGAGCCATCTCGAGAACAAA
GAGTGTGTAATTGTCTGCCCAGTAGTTGTATGT
   
Sequence Name 84-K034733-022-055-D01C-FB05
GenBank Accession LN514382 [GenBank]
Sequencing primer gene specific primer, FB05: 5'-GACATACACTTACTGGGCAGACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K034733-022-055-D01C-FB05
CTCTGATGGGATTCGCTGAGCACCGGAGGTTACGGACTGGTACAACCCAGGATCTATGGG
AAAACAGTACTTCTTGGGGTTAGAGAAGGGTTTGGCCGGTTCAGGTAACCCGGCTTACCC
CGGTGGACCTTTCTTCAACCCTCTTGGGTTTGGGAAAGATGAGAAGTCACTGAAGGAGTT
GAAACTCAAGGAGGTCAAGAACGGTAGACTGGCTATGCTCGCCATCCTCGGTTACTTTAT
CCAAGGACTAGTGACCGGTGTGGGACCTTATCAGAACCTGCTTGATCACTTGGCTGATCC
CGTCAACAACAACGTCTTGACCAGCCTCAAGTTCCACTGAGGTGATCATCATCTCATGTG
TTTTTATGTCTCTTGTATCGTACAATATTTCCATCTTTCGCTACCATTGGCTTCTTCGTA
TTGTAACACCCTGTGTAAACATATTCGTGTTTTGTAAGAAATACTCAAACCAAACAAAGC
GGTTAGTATTCAATTGCAATTGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATT
TTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTAT
TCTAATTCGGAAATCTTTATTTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11