Sequences from confirmation amplicon


Confirmation sequences for line 055E02 and hit At3g01311 are shown.
Line ID 055E02
Locus At3g01311 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:103215
Primers used for PCR NA17 - 8474, show primer details
   
Sequence Name 02-K101668-0022-055-E02C-8409
GenBank Accession LN486970 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K101668-0022-055-E02C-8409
TTCCGGAATGAAGCTTTACAATTGAATATATCCTGCACATCATCGTCTTTCCTTCTTCTT
GCGGTACTAAACTTACCTCCTTCTTCATTGTTACCATTATCTATAATCAAAATATTGTTT
ATGCATTCCTAATTTTTTTCAATTGAGGATTTCGATTTCCAATTAAAACTTTAGGTATAT
TCGAGACATTGGGAGTCCAGAGTTGTTGTTTTTCCGATTTCTTTTGGTCGGAACTCTAAT
GAAAAATCCGACACATAGTTTTCTTGAAAGTCTCATTATTCGTCACGTCAGCACAAACTT
TTATTCATCCCAAGTTTAACTATTTTAATACAGCCTCTAATTGTGTTTTTGATTTTTTGA
TTTTTTTTTTTTCCCAGAAATTGACAAGTGATTGTCAATAATATATTATCAAATTATAAC
CAACTTGGGAAGAAGAAAAA
   
Sequence Name 02-K101674-0022-055-E02C-NA17
GenBank Accession LN486971 [GenBank]
Sequencing primer gene specific primer, NA17: 5'-TTTTCTTCTTCCCAAGTTGGTTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K101674-0022-055-E02C-NA17
GAAAANAAGGTGGGGGGGATTTCATGAGAGTTCCGACCAAAAGAATTCGGAAAAACAACA
GCTCTAGGACTCCCAAGGTCTCGAATATACCTAAAGTTTAAATTGGAAACCGAAATCCTC
AATTGAAAAAAATGAGGAAGGGATAAACAATATTTTGATTATAGATAAGGGTAACAAGGA
AGAAGGAGGTAAGTTTAGTACCGCAAGAAGAAGGAAAGACGATGATGCGCAGGAAATATC
CAATTGGAAAGGGCTTCAGGTCCTGTTAATCCACATGGATCAGCAATGAGGATGATGGTC
AACATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGAAGATGTC
CGCATCGTGATAAAAAAAAGCTCGAAACTTCATGTTTTAAATGG
Primers used for PCR NA18 - 3144, show primer details
   
Sequence Name 03-K101668-0022-055-E02C-3144
GenBank Accession LN487424 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K101668-0022-055-E02C-3144
TATCCTCCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGG
TACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTCTACACC
AAATCATAACATCTTCCATCTCTGTTTCTTCCTCAAGCTTCTTCTTTAGTTCTATCAACT
CGCTTCCATGGAATATCAATGATTTTTCTCCTATTTCCTTATTTATGTTCCCACTATCAT
CTCCGGTCTCGTAATATATTAGCCTTCCTTCGGACTTGAGTGGTAAACTCACAATAAACG
AATCATCTGACTGATCATGTATCGATGAATGTTTAGTACTAGTATTTTCAGAAGAATGGT
ATTGGCACGTATGTGTAGTGTATATAGTAGTAGTACCTCAATCCGTGAAAAAGTGGAAGA
ACTGTGGGTAGACTCTGCATCATCTTTGATAACTCGAATCTGAAGAACGTCAACAGTCCA
CATGACCCAATCTTGTGTGACCGTACGACTAGGAATATCGTGTGTGATAGAGTTTCTCCA
CGGTGGTACACCTTTGTTTGCCCTCAGATATTGTCCGTAGCGACTCTTGAACCGTACGTG
TACACCATCGTCTGATAGCGGCTCCCACTCCGCAGATGAATCCNACTTTTTCGGTAGAGT
TTGAAGTACCTTGTGAATATGAAACACACACGGCTTGTTTAGC
   
Sequence Name 03-K101674-0022-055-E02C-NA18
GenBank Accession LN487425 [GenBank]
Sequencing primer gene specific primer, NA18: 5'-GAAATGTTTCTTGCTAAACAAGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K101674-0022-055-E02C-NA18
TCAACTCTACCCGAAAAGTTGGATTCATCTGCGGAGTGGGAGCCGCTATCAGACGATGGT
GTACACGTACGGTTCAAGAGTCGCTACGGACAATATCTGAGGGCAAACAAAGGTGTACCA
CCGTGGAGAAACTCTATCACACACGATATTCCTAGTCGTACGGTCACACAAGATTGGGTC
ATGTGGACTGTTGACGTTCTTCAGATTCGAGTTATCAAAGATGATGCAGAGTCTACCCAC
AGTTCTTCCACTTTTTCACGGATTGAGGTACTACTACTATATACACTACACATACGTGCC
AATACCATTCTTCTGAAAATACTAGTACTAAACATTCATCGATACATGATCAGTCAGATG
ATTCGTTTATTGTGAGTTTACCACTCAAGTCCGAAGGAAGGCTAATATATTACGAGACCG
GAGATGATAGTGGGAACATAAATAAGGAAATAGGAGAAAAATCATTGATATTCCATGGAA
GCGAGTTGATAGAACTAAAGAAGAAGCTTGAGGAAGAAACAGAGATGGAAGATGTTATGA
TTTGGTGTAGAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGG
GTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGGAGG
ATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATAT

Last Updated on 06.12.2018 16:11