Sequences from confirmation amplicon


Confirmation sequences for line 055E09 and hit At2g29050 are shown.
Line ID 055E09
Locus At2g29050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12479063
Primers used for PCR DL05 - 8474, show primer details
   
Sequence Name 07-K016384-022-055-E09C-8409
GenBank Accession LN489038 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K016384-022-055-E09C-8409
AATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAAACTGAAAGACTTGCTT
TTTATTTACTAATTCTCATTTTCTTTGACAGTACGCATTGGATTGCTTTATATGATATCT
GGATTTGGTGGAAGTTTGTTATCTTCTCTATTTAACCGGGCTGGTATATCCGTTGGTGCC
TCTGGAGCATTATTCGGCTTGCTTGGTGCTATGCTTTCAGAGCTTCTCACTAATTGGACT
ATTTACGCTAATAAGGTAGCCAAATCTTCCCTTGTTAAACAAGCCGCACTTTCGATGAAT
GATGTGTCAATAATGTCATTGGTTTTTCTACATTTGCAGTTTGCAGCTCTTTTGACACTC
ATCTTCATCATAGCCATTAATTTAGCAGTAGGTATTCTTCCACATGTAGACAACTTTGCC
CATCTTGGAGGATTCACATCAGGGTTTCTTCTAGGCTTTGTTTTCTTGATACGTCCTCAG
TATGGATACTTCAACCAGCGGAACAATCCTCGGGGTTATGCTGCACCGTCTGCTAAATCC
AAACACAAGCCATACCAATACGTTCTTTGGATCACCTC
   
Sequence Name 08-K016384-022-055-E09C-DL05
GenBank Accession LN489503 [GenBank]
Sequencing primer gene specific primer, DL05: 5'-AAAAGCACTAAAGAGGTGATCCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K016384-022-055-E09C-dl05
TATTGGTATGGCTTGTGTTTGGATTTAGCAGACGGTGCAGCATAACCCCGAGGATTGTTC
CGCTGGTTGAAGTATCCATACTGAGGACGTATCAAGAAAACAAAGCCTAGAAGAAACCCT
GATGTGAATCCTCCAAGATGGGCAAAGTTGTCTACATGTGGAAGAATACCTACTGCTAAA
TTAATGGCTATGATGAAGATGAGTGTCAAAAGAGCTGCAAACTGCAAATGTAGAAAAACC
AATGACATTATTGACACATCATTCATCGAAAGTGCGGCTTGTTTAACAAGGGAAGATTTG
GCTACCTTATTAGCGTAAATAGTCCAATTAGTGAGAAGCTCTGAAAGCATAGCACCAAGC
AAGCCGAATAATGCTCCAGAGGCACCAACGGATATACCAGCCCGGTTAAATAGAGAAGAT
AACAAACTTCCACCAAATCCAGATATCATATAAAGCAATCCAATGCGTACTGTCAAAGAA
AATGAGAATTAGTAAATAAAAAGCAAGTCTTTCAGTTTCAGGATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
TATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCG
AAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTC

Last Updated on 06.12.2018 16:11