Sequences from confirmation amplicon


Confirmation sequences for line 055F01 and hit At1g80610 are shown.
Line ID 055F01
Locus At1g80610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:30303044
Primers used for PCR 03ve - 8474, show primer details
   
Sequence Name 67-K118051-0022-055-F01C-03ve
GenBank Accession LN511900 [GenBank]
Sequencing primer gene specific primer, 03ve: 5'-GTAGCTTGTGGGTATTTTGGTCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K118051-0022-055-F01C-03ve
GAAATACCACCAGCGTGGACACAACTCCAATTTAGGCAATGATGTTTTTAGTCTTTTCTT
TTCCAAGAAACTCAATCTATCTTTCTCGATTTCTTTTGTTGTCTTCTTGGAAACACTTGT
TAGATTGTTTTTGAGTTTCAAACAAACAGGTGATAAATTCGGTGATCTCTGCTTTTTGCT
CTTTTCTTTTGGCAACAAAAGAGTCACGTTTTCCCACTTTTGTGCATCTCAGTCATCTTT
CTCTCTCTCTCTCTATGCTGCTTTGCGGAAAGACCACAAAGCCCTCACATCAAGGGAGAG
GGGAGAGGTCTTCTGGCGTTATTTGACCCGAAGACAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 31-K118050-0022-055-F01C-8409
GenBank Accession LN500333 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K118050-0022-055-F01C-8409
CTCTCCCTCTCCTTGATGTGAGGGCTTTGTGGTCTTTCCGCAAAGCAGCATAGAGAGAGA
GAGAGAAAGATGACTGAGATGCACAAAAGTGGGAAAACGTGACTCTTTTGTTGCCAAAAG
AAAAGAGCAAAAAGCAGAGATCACCGAATTTATCACCTGTTTGTTTGAAACTCAAAAACA
ATCTAACAAGTGTTTCCAAGAAGACAACAAAAGAAATCGAGAAAGATAGATTGAGTTTCT
TGGAAAAGAAAAGACTAAAAACATCATTGCCTAAATTGGAGTTGTGTCCACGCTGGGGGT
ATTTCGGTCAACGAAAAGTCGGTGTACCTACATGATCAGCTTAAGAACACGATCGTGGAT
CCTAAAATGACCAAAATACCANCAAGCTA

Last Updated on 06.12.2018 16:11