Sequences from confirmation amplicon


Confirmation sequences for line 055F02 and hit At3g09820 are shown.
Line ID 055F02
Locus At3g09820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3013682
Primers used for PCR KM24 - 3144, show primer details
   
Sequence Name 68-K032612-022-055-F02C-3144
GenBank Accession LN511935 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K032612-022-055-F02C-3144
CACTATCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGG
GTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAACCT
TTCTGCTCCATTCATCTGTGAATTCTTCAAAGATGTTCAGGAGAAGTGCCTGCCGTAAGC
ACATCCTAATCTCTCGATCCATTTATTCCATCTTATAATTGCCTAAACTAATCACTGGCG
ATATACTGAAAAGCAAGACGCTGCATCTAATGTCTTGCTTGGACCAGGTACATGGACTAT
ATTTTTGGCAATGAGACCGAGGCAAGAACCTTCTCCAGGGTTCACGGCTGGGAGGTAACT
GTCGAAATTTCACCCTGTATTGTTTGAAGAAAGACAGTTTATTAATCTGTTTGGTTATAT
TTTATACAGACTGATGATGTTGAGCAAATAGCCATCAAGATGTCCCAGTTACCCAAAGCC
TCTGGAACATACAAGAGGACTACTGTGATTACACAAGGCGCAGATCCTGTGGTTGTCGCT
GAGGATGGAAAAGTGA
   
Sequence Name 68-K032612-022-055-F02C-KM24
GenBank Accession LN511936 [GenBank]
Sequencing primer gene specific primer, KM24: 5'-ACTGGGTACTTCTTCACTTTTCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K032612-022-055-F02C-KM24
GCCTGTGTATCACAGTAGTCCTCTTGTATGTTCAGAGGCTTTGGGTAACTGGGACATCTT
GATGGCTATTTGCTCAACATCATCAGTCTGTATAAAATATAACCAAACAGATTAATAAAC
TGTCTTTCTTCAAACAATACAGGGTGAAATTTCGACAGTTACCTCCCAGCCGTGAACCCT
GGAGAAGGTTCTTGCCTCGGTCTCATTGCCAAAAATATAGTCCATGTACCTGGTCCAAGC
AAGACATTAGATGCAGCGTCTTGCTTTTCAGTATATCGCCAGTGATTAGTTTAGGCAATT
ATAAGATGGAATAAATGGATCGAGAGATTAGGATGTGCTTACGGCAGGCACTTCTCCTGA
ACATCTTTGAAGAATTCACAGATGAATGGAGCAGAAAGGTTCAAACACTGATAGTTTAAA
CCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAA
CTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACG
TCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTT
TCCACGATGCTCCTCGTGGGTG

Last Updated on 06.12.2018 16:11