Sequences from confirmation amplicon


Confirmation sequences for line 055G09 and hit At5g22480 are shown.
Line ID 055G09
Locus At5g22480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7452445
Primers used for PCR 61zk - 8474, show primer details
   
Sequence Name 62-K128427-0022-055-G09C-61zk
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 61zk: 5'-AACCATCAAATTCTATGAGCGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K128427-0022-055-G09C-61zk
GTTGCTAACCCATCTCGGCTGGACATCAGAAGGAAGCCTTGGCGCACCTTCTACTAAAAC
AGCTTATGTACCTAATGGAACAATCGGAGCAACTGCTGGTCATCGCGCGATTGCTCAGAG
TAATAGCACTGATATTTCCGACAACTTGTTCAGATACTCTGCGCCTGAAGAGGTAACTTC
TGACGCTGTAACTTTTGAAATAGTTTTTGTTGGATGTTCTGTTTCAAGGTTATTATTGCT
CGAGAAAATAATCAGCGCCCAAGGTTGTATTTGTGAGAGTGAATACTTAGATCATAGTAA
TATAATTTCATATCCCGTTACAATTTCTCTAACAGGTGATGACTTTCCCTTCAACTTGCG
GAGCATGTACGGAGCCGTGTGAGACACGGATGTTCGTGACTAGTATCCTTTCAAAGTTTG
GACCCGTTCATTTCTTGGTTTTCCGCAACAAGTTTCCATATTTGTGTTTGTTGGTGAATT
CCTAGATGTTGTTTACCACTTAACAGAAAATAGAAATCCCGTACTTTCAGGAAGTTATTG
TCATGGCATCTACATGTGACAGTTGTGGCTATCGTAATTCTGAGGTTGGGTTTCGTTGGT
TTGATCTTGTTAGAGAATTTCTGATATATGCCTGGTGTTTAGGTTACTAACTTTTTTTGG
GTTATCGTGAGCAGTTGAAGCCTGGTGGTGCAATTCCTGAAAAGGGAAAGAAGATTACTC
TCTCTGTGAGGAACATTACAGACCTTAGCCGAGATGTTATCAAGGTCAGTTTTCGCTTCA
TTTTGTTTTGCACCAGTTCTCTCAGTGGCGGATCAATAGATATTTCCTCATATTATTCTA
TGTACCTTAAAAGTAGCTAAGATTGTCACAGTTTAAGTTTACAGAATGTATGTAGT
   
Sequence Name 46-K128426-0022-055-G09C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K128426-0022-055-G09C-8409
CATTTACAATTGAATAACAAGGATAAAGGATAATGTTGAGATTTTGAAGGGAATGTAGAG
ATTTTGAAGGGCACACTCACTTTCTCTGATCTGTGTAACCAACCCTTCAACTGTTGTTAC
CATTCCACCAAGTGTACCACCAGCTAGCTCCAGATCAAGTTCTGGGATTATCACTCCTGC
AGTGTCCGACTAATACACACGATCAAAATTTACCATTAATGACATAGTAGAAGGTGTTGC
TATAAAAATCTAAAGGATCATAAGGTTGTCTACTATAATATTGCCATCAGATGTGTTATA
TAGCCACACCAAAGGCCTAGAAAGATCAAACAAGCCTGTTTTACATCTGATCTCGAAGTT
TCGACACTTATCTGCCACTACCTAGATATATTATTTTCTGCCAGGACATGAGAATACCAT
ATATAATTTGGCAGCGAGCAAGATTCCTTGTCAAACCTTTACCCAACTTAAACTACATAC
ATTCTGTAAACTTAAACTGTGACAATCTTAGCTACTTTTAAGGTACATAGAATAATATGA
GGAAATATCTATTGATCCGCCACTGAGAGAACTGGTGCAAAACAAAATGAAGCGAAAACT
GACCTTGATAACATCTCGGCTAAGGTCTGTAATGTTCCTCACAGAGAGAGTAATCTTCTT
TCCCTTTTCAGGAATTGCACCACCAGGCTTCAACTGCTCACGATAACCCAAAAAAAGTTA
GTAACCTAAACACCAGGCATATATCAGAAATTCTCTAACAAGATCAAACCAACGAAACCC
AACCTCAGAATTACGATAGCCACAACTGTCACATGTAGATGCCATGACAATAACTTCCTG
AAAGTACGGGATTTCTATTTTCTGTTAAGTGGTAAACAACATCTAGGAATTCACCNACAA
ACACAAATAT

Last Updated on 06.12.2018 16:11