Sequences from confirmation amplicon


Confirmation sequences for line 055G11 and hit At4g29180 are shown.
Line ID 055G11
Locus At4g29180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14388739
Primers used for PCR KE32 - 8474, show primer details
   
Sequence Name 32-K100995-0022-055-G11C-8409
GenBank Accession LN500507 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K100995-0022-055-G11C-8409
GAGCCATTCCCGGTTATTGATTTGTTTTCCTTGGCAGGGTTGGAGTATCTTCATCATGGC
TGCAGGCCACCAATAGTACACAGAGACGTTAAAACAGCAAACATTCTTTTAAACGATAAC
TTGGAAGCCAAGATCGCTGATTTCGGGCTTTCTAAGGTCTTCCCTGAGGATGATCTCAGC
CACGTTGTGACTGCTGTCATGGGCACTCCTGGCTATGTCGATCCTGAGTAAGTTTCCTTA
ACACATCACCACTTTTAATCTTTACCATTTTGGTCATGTTGGTCTCATCATAAACTGGTT
TCAAAATAGGTACTACAACACGTTTAAGCTAAACGAGAAGAGCGACGTGTACAGCTTTGG
GATCGTCCTCCTTGAACTCATAACCGGCAAGAGATCCATAATGAAAACTGACGACGGAGA
AAAAATGAACGTTGTTCACTACGTCGAGCCTTTCCTCAAAATGGGAG
   
Sequence Name 32-K100999-0022-055-G11C-KE32
GenBank Accession LN500508 [GenBank]
Sequencing primer gene specific primer, KE32: 5'-GACACCATCTATGTCTCCCATTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K100999-0022-055-G11C-KE32
CGTTCTTTTTTCTCCGTCGTCAGTTTTCATTATGGATCTCTTGCCGGTTATGAGTTCAAG
GAGGACGATCCCAAAGCTGTACACGTCGCTCTTCTCGTTTAGCTTAAACGTGTTGTAGTA
CCTATTTTGAAACCAGTTTATGATGAGACCAACATGACCAAAATGGTAAAGATTAAAAGT
GGTGATGTGTTAAGGAAACTTACTCAGGATCGACATAGCCAGGAGTGCCCATGACAGCAG
TCACAACGTGGCTGAGATCATCCTCAGGGAAGACCTTAGAAAGCCCGAAATCAGCGATCT
TGGCTTCCAAGTTATCGTTTAAAAGAATGTTTGCTGTTTTAACGTCTCTGTGTACTATTG
GTGGCCTGCAGCCATGATGAAGATACTCCAACCCTGCCAAGGAAAACAAATCAATAAACC
GGGAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC

Last Updated on 06.12.2018 16:11