Sequences from confirmation amplicon


Confirmation sequences for line 055H02 and hit At2g31220 are shown.
Line ID 055H02
Locus At2g31220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13303882
Primers used for PCR H10b - 8474, show primer details
   
Sequence Name 19-K014701-022-055-H02C-8409
GenBank Accession LN494538 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K014701-022-055-H02C-8409
CTGTAGATTTCCCGGACATGAAGCCATTTACAATTGAAAATGAACCTCATTTTTCTTTTT
CTTCAATGATCGTTTCTTCGACTTAAAGAACCTCATTCCAAATCCCACAAAGGTATATAC
TTTACTTTGCTTTGATTTCAATTCATGTTGTTAATGTGTATGAGGAGACTATGAAATGAG
AGACATTGTTGGTTTAATCTCTGTAGATTGATAGAGCATCGATAGTTGGAGAGGCGATAG
ATTACATCAAAGAGCTTTTAAGGACAATAGAGGAGTTTAAGATGCTTGTGGAGAAGAAGA
GATGTGGGAGATTCAGGAGCAAGAAGAGAGCTAGAGTTGGTGAAGGAGGAGGAGGAGAAG
ATCAAGAAGAAGAAGAAGACACTGTGAATTACAAGCCACAGAGCGAAGTAGACCAGTCTT
GCTTCAACAAGAACAACAACAACTCACTGAGATGTTCATGGCTTAAGAGGAAATCGAAAG
TCACTGAGGTCGATGTACGTATAATCGATGATGAAGTAACCATCAAGCTTGTTCAGAAGA
AGAAGATTAACTGTTTGTTGTTCACCACCAAAGTTCTTGATCAGCTTCAGCTAGATCTTC
ACCATGTTGCAGGTGGACAGATTGGTGAACATTACAGCTTCTTGTTCAACACTAAGGTAA
TGAAAATGTCTCACTAATATAGTTCTGTTATTAACTCCAAACCAATAAACTAATCTTT
   
Sequence Name 20-K014701-022-055-H02C-H10b
GenBank Accession LN494988 [GenBank]
Sequencing primer gene specific primer, H10b: 5'-CCGTTGCTAGGAACAGCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K014701-022-055-H02C-H10b
CATGTACTGCTTCTCCCGACCTCCATCAATGTGTCTGCAATTCCACTTGCATACACACAA
GATCCTTCACAAATCTGCCTCAAACCAAATTGAAAAAGATTAGTTTATTGGTTTGGAGTT
AATAACAGAACTATATTAGTGAGACATTTTCATTACCTTAGTG
Primers used for PCR FM32 - 8474, show primer details
   
Sequence Name 53-K029301-022-055-H02C-8409
GenBank Accession LN508043 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K029301-022-055-H02C-8409
CATTTACAATTGAAAATGAACCTCATTTTTCTTTTTCTTCAATGATCGTTTCTTCGACTT
AAAGAACCTCATTCCAAATCCCACAAAGGTATATACTTTACTTTGCTTTGATTTCAATTC
ATGTTGTTAATGTGTATGAGGAGACTATGAAATGAGAGACATTGTTGGTTTAATCTCTGT
AGATTGATAGAGCATCGATAGTTGGAGAGGCGATAGATTACATCAAAGAGCTTTTAAGGA
CAATAGAGGAGTTTAAGATGCTTGTGGAGAAGAAGAGATGTGGGAGATTCAGGAGCAAGA
AGAGAGCTAGAGTTGGTGAAGGAGGAGGAGGAGAAGATCAAGAAGAAGAAGAAGACACTG
TGAATTACAAGCCACAGAGCGAAGTAGACCAGTCTTGCTTCAACAAGAACAACAACAACT
CACTGAGATGTTCAT
   
Sequence Name 54-K029301-022-055-H02C-FM32
GenBank Accession LN508366 [GenBank]
Sequencing primer gene specific primer, FM32: 5'-TTTCCTCTTAAGCCATGAACATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K029301-022-055-H02C-FM32
CAGACTGGTCTACTTCGCTCTGTGGCTTGTAATTCACAGTGTCTTCTTCTTCTTCTTGAT
CTTCTCCTCCTCCTCCTTCACCAACTCTAGCTCTCTTCTTGCTCCTGAATCTCCCACATC
TCTTCTTCTCCACAAGCATCTTAAACTCCTCTATTGTCCTTAAAAGCTCTTTGATGTAAT
CTATCGCCTCTCCAACTATCGATGCTCTATCAATCTACAGAGATTAAACCAACAATGTCT
CTCATTTCATAGTCTCCTCATACACATTAACAACATGAATTGAAATCAAAGCAAAGTAAA
GTATATACCTTTGTGGGATTTGGAATGAGGTTCTTTAAGTCGAAGAAACGATCATTGAAG
AAAAAGAAAAATGAGGTTCATTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAAT
TCGGAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11