Sequences from confirmation amplicon


Confirmation sequences for line 055H09 and hit At4g38390 are shown.
Line ID 055H09
Locus At4g38390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17976497
Primers used for PCR MR98 - 8474, show primer details
   
Sequence Name 29-K017447-022-055-H09C-MR98
GenBank Accession LN499079 [GenBank]
Sequencing primer gene specific primer, MR98: 5'-GGTGGATATTCTTGACTGCTCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K017447-022-055-H09C-MR98
TTTAGAAGCTCCTTGATGATCTTCACGTCTTTGGAGAGATGCGATATGAACCAATCAACA
TCAAATATATCCTCAAAGTTGCTGCGTACCACAAAACAATTCAAAACAAAACCTGTGTGT
TAGAACTTCGAACCAACAAATTTTTAAAATGGGAACAGAGAGGGACAGGGCAGGGACCTG
GTGTCCTTCCAGTACGACTTCTGGTCAAGTTTAGGGACCACAAGAGTGGCGTTTAGAATG
TATGCCGCTACTACAGCATCTATTATCTGCAGTAGAAACCAAACTTAATCAGAAATATAT
ATATGAATTAACCACTTGAAGAAGCATCATAACACACAAGGAGGAACTCACCCCTGTCCG
CTGTTGGTTTAACCCTCCGCTTGTTGCGATCAGTAAATACCGATTGGTTTGGCTTCTCGT
ATCCGTAACTGCCACAATTTCAATTTAAGTCAATTTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAG
TACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGGCGAAAGCAATAAAC
AAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
   
Sequence Name 30-K017447-022-055-H09C-8409
GenBank Accession LN499515 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K017447-022-055-H09C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAAATTGACTTAAATTGAAATTGTGGCA
GTTACGGATACGAGAAGCCAAACCAATCGGTATTTACTGATCGCAACAAGCGGAGGGTTA
AACCAACAGCGGACAGGGGTGAGTTCCTCCTTGTGTGTTATGATGCTTCTTCAAGTGGTT
AATTCATATATATATTTCTGATTAAGTTTGGTTTCTACTGCAGATAATAGATGCTGTAGT
AGCGGCATACATTCTAAACGCCACTCTTGTGGTCCCTAAACTTGACCAGAAGTCGTACTG
GAAGGACACCAGGTCCCTGCCCTGTCCCTCTCTGTTCCCATTTTAAAAATTTGTTGGTTC
GAAGTTCTAACACACAGGTTTTGTTTTGAATTGTTTTGTGGTACGCAGCAACTTTGAGGA
TATATTTGATGTTGATTGGTTCATATCGCATCTCTCCAAAGACGTGAAGATCATCAAGGA
GCTTCCTAAAGAAGAGCAGTCAA
Primers used for PCR FM33 - 8474, show primer details
   
Sequence Name 15-K029742-022-055-H09C-8409
GenBank Accession LN492842 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K029742-022-055-H09C-8409
GCCATTTTTGTCTATTTACGCAAATGGATCAAATCTTTACCTTGAAAGGTGTCAGTTGCA
TTACTGCAGGCATAGTACAAGTTGGACAATCTCGAGCTCCATAACTGGCGATCCGACCTT
CCGCCATTTTCCTGTGACACGCATTAATGATCATGAATGACCTGGAAAGTTTGTGTAAAA
CCTCAATGAAACAAAAAAGAGTTTACGTACGGGTACACGAAATGGAGTGGTTGGATTGGG
TTCGACGGCCACTTGATTGTTATGATGGATCAATGGAGGAGGAGAGAGGGTGGAGAATGA
GAAGAGGATGAGGAGGAGACAGCCGGAGACGGCAGATACCAGCGGAAATAGACGGTGACG
GCGGCGAAGGAGAAGGAGTAACTGGTGGCGAATACCGGCGATGACCAGCTTTTTCTTGTC
GGGTTCAGCGCCATTATCCCTTTTCTTCACNTTCAAAG
   
Sequence Name 16-K029742-022-055-H09C-FM33
GenBank Accession LN493311 [GenBank]
Sequencing primer gene specific primer, FM33: 5'-CTCTTTGAAGTTGAAGAAAAGGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K029742-022-055-H09C-FM33
AGCTGGTCATCGCCGGGTATTCGCCACCAGTTACTCCTTCTCCTTCGCCGCCGTCACCGT
CTATTTCCGCTGGTATCTGCCGTCTCCGGCTGTCTCCTCCTCATCCTCTTCTCATTCTCC
ACCCTCTCTCCTCCTCCATTGATCCATCATAACAATCAAGTGGCCGTCGAACCCAATCCA
ACCACTCCATTTCGTGTACCCGTACGTAAACTCTTTTTTGTTTCATTGAGGTTTTACACA
AACTTTCCAGGTCATTCATGATCATTAATGCGTGTCACAGGAAAATGGCGGAAGGTCGGA
TCGCCAGTTATGGAGCTCGAGATTGTCCAACTTGTACTATGCCTGCAGTAATGCAACTGA
CACCTTTCAAGGTAAAGATTTGATCCATTTGCGTAAATAGACAAAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAAC
AAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11