Sequences from confirmation amplicon


Confirmation sequences for line 057C03 and hit At1g16220 are shown.
Line ID 057C03
Locus At1g16220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5550386
Primers used for PCR 06po - 8474, show primer details
   
Sequence Name 54-K115121-0022-057-C03C-06po
GenBank Accession LN508573 [GenBank]
Sequencing primer gene specific primer, 06po: 5'-CTTTAGGTGAATCAGCAAGAATCC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K115121-0022-057-C03C-06po
CTTCAGACGAGCCTGAGGTTGCGCGTGTGTGGTTACCGAACAGCGATTCACCTGGTTTAG
CAATGGCTCGAGCTTTTGGGGACTTCTGCCTTAAAGATTATGGTCTAATCTCTGTCCCGG
ATATCAACTACCATAGACTCACTGAAAGAGATCAGTATATTATTCTAGCTACTGACGGGG
TACACATTACTCTTCTTATTCTTCTCACTGGTTACTATCGAAATCTTTGGAGCTAAAGTG
TCTCTAATGCTAGGTATGGGATGTTTTGTCGAACAAAGAAGCTGTTGATATTGTGGCTTC
AGCTCCTAGTCGAGATACTGCAGCTCGTGCTGTAGTCGACACGGCGGTTCGAGCATGGAG
GCTTAAGTATCCTACTTCCAAGAATGATGATTGTGCTGTAGTATGTCTCTTTCTTGAAGA
CACTTCAGCAGGAGGAACCGTGGAAGTTTCGGAAACCGTTAATCATTCTCATGAAGAGTC
TACAGAGAGTGTCACCATAACATCAAGCAAAGATGCCGACAAGAAGGAAGAGGCTTCAAC
AGAAACAAATGAGACTGTACCGGTTTGGGAGATAAAAGAAGAAAAGACGCCTGATGAAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGGTT
ATT
   
Sequence Name 06-K115120-0022-057-C03C-8409
GenBank Accession LN488935 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K115120-0022-057-C03C-8409
TTTCTCAGGCGTCTTTTCTTCTTTATCTCCAAACCGGTACAGTCTCATTTGTTTCTGTTG
AAGCCTCTTCCTTCTTGTCGGCATCTTTGCTTGATGTTATGGTGACACTCTCTGTAGACT
CTTCATGAGAATGATTAACGGTTTCCGAAACTTCCACGGTTCCTCCTGCTGAAGTGTCTT
CAAGAAAGAGACATACTACAGCACAATCATCATTCTTGGAAGTAGGATACTTAAGCCTCC
ATGCTCGAACCGCCGTGTCGACTACAGCACGAGCTGCAGTATCTCGACTAGGAGCTGAAG
CCACAATATCAACAGCTTCTTTGTTCGACAAAACATCCCATACCTAGCATTAGAGACACT
TTAGCTCCAAAGATTTCGATAGTAACCAGTGAGAAGAATAAGAAGAGTAATGTGTACCCC
GTCAGTAGCTAGAATAATATACTGATCTCTTTCAGTGAGTCTATGGTAGTTGATATCCGG
GACAGAGATTAGACCATAATCTTTAAGGCAGAAGTCCCCAAAAGCTCGAGCCATTGCTAA
ACCAGGTGAATCGCTGTTCGGTAACCACACACGCGCAACCTCAGGCTCGTCTTGTAAGGC
AAACACTCTACCTTTACATCTATGGATTCTTGCTGATCAACCTAAA

Last Updated on 06.12.2018 16:11