Sequences from confirmation amplicon


Confirmation sequences for line 057E08 and hit At3g13930 are shown.
Line ID 057E08
Locus At3g13930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4599541
Primers used for PCR 06de - 8474, show primer details
   
Sequence Name 31-K109674-0022-057-E08C-06de
GenBank Accession LN500203 [GenBank]
Sequencing primer gene specific primer, 06de: 5'-GGTGCATCTTACTTCTTTTCTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K109674-0022-057-E08C-06de
CATTATGAAACCTTTTCTTTTTCGAAATTGCAGGCTGCTGCACTGGCTCTTCGAAAAGTT
CCTCAGTGCAATAGTTCATGGACAGATGAATACATCCGCCAGTAAGTACATATTCTGTTT
CAGAGGTTCATGAAAACTTTGCAAATCATCTAGTTTCTTGGTTCTTATTTTCCTCCTTTT
ATGTACAGATTTAAAAATGTGAACATCAATGTCGCTGTACAAACAGAAAATGGGCTCTAC
GTTCCTGTGGTCAAGGTTAGGGAGCTCTTGATACAGTTATTAACCTTCCTCTGCAGAAAC
TTGAGATTTCTGAAAAACTCAGTTCATCTAATTGCTTGATTTCAGGACGCGGACAAGAAA
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 15-K109673-0022-057-E08C-8409
GenBank Accession LN493015 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K109673-0022-057-E08C-8409
TCTTCTTGTCGCGTCCTGAATCAAGCAATTAGATGAACTGAGTTTTTCAGAAATCTCAAG
TTTCTGCAGAGGAAGGTTAATAACTGTATCAAGAGCTCCCTAACCTTGACCACAGGAACG
TAGAGCCCATTTTCTGTTTGTACAGCGACATTGATGTTCACATTTTTAAATCTGTACATA
AAAGGAGGAAAATAAGAACCAAGAAACTAGATGATTTGCAAAGTTTTCATGAACCTCTGA
AACAGAATATGTACTTACTGGCGGATGTATTCATCTGTCCATGAACTATTGCACTGAGGA
ACTTTTCGAAGAGCCAGTGCAGCAGCCTGCAATTTCGAAAAAGAAAAGGTTTCATAATGG
AGAACAAAATGAGATTTAATTAGACTTGCAAGAAAAGAAGTANGATGCAC

Last Updated on 06.12.2018 16:11