Sequences from confirmation amplicon


Confirmation sequences for line 057F11 and hit At1g53300 are shown.
Line ID 057F11
Locus At1g53300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19880486
Primers used for PCR 20do - 8474, show primer details
   
Sequence Name 29-K109936-0022-057-F11C-20do
GenBank Accession LN499332 [GenBank]
Sequencing primer gene specific primer, 20do: 5'-CGGACTCTGCTACAACTTCTTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K109936-0022-057-F11C-20do
TCCTCCTCTGCTACTTCTCCAGCTCCGACGTCTCCGGCGAATGTACTTCCCACCGGAAAC
ATTTGTCCTTCTGGGAAGATCCAAATCACTGGAATGACTCAAAGCCGTTCAAGAAGCGAT
GTTCTTGGATCTGGTACTGGAACTTATGGACATGGAAGTATAATGCGAGGAGGTGGTATA
TCTCCGGCCAAACCTACCAACACCGGAGGAGGATCCAACTCGCCGGTAAATGTCGGTAGC
TCTAGTAGAAGTAGTAGTACTGTTGCTACGGGAGAGACTCCTATATGGAAAAAAGCGATT
TTAGGTTCGGATTCAGAGGAAGTGAAGAGAGTAGGGAATGAAATGTATAGGAAAGGTTTG
TTTAATGAGGCTTTGAAGTTGTATGATAGAGCAATTGTATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 13-K109935-0022-057-F11C-8409
GenBank Accession LN492084 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K109935-0022-057-F11C-8409
CATTTACATTGAATACATTGCTCTATCATACAACTTCAAAGCCTCATTAAACAAACCTTT
CCTATACATTTCATTCCCTACTCTCTTCACTTCCTCTGAATCCGAACCTAAAATCGCTTT
TTTCCATATAGGAGTCTCTCCCGTAGCAACAGTACTACTACTTCTACTAGAGCTACCGAC
ATTTACCGGCGAGTTGGATCCTCCTCCGGTGTTGGTAGGTTTGGCCGGAGATATACCACC
TCCTCGCATTATACTTCCATGTCCATAAGTTCCAGTACCAGATCCAAGAACATCGCTTCT
TGAACGGCTTTGAGTCATTCCAGTGATTTGGATCTTCCCAGAAGGACAAATGTTTCCGGT
GGGAAGTACATTCGCCGGAGACGTCGGAGCTGGAGAAGTAGCAGAGGAGGAGGAGGAGGA
GAGAAGTGGTTGTGAATGAGAAGAAGAAGAAGTTGTAGC

Last Updated on 06.12.2018 16:11