Sequences from confirmation amplicon


Confirmation sequences for line 057H05 and hit At2g30200 are shown.
Line ID 057H05
Locus At2g30200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12883287
Primers used for PCR 02yp - 8474, show primer details
   
Sequence Name 82-K121557-0022-057-H05C-02yp
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 02yp: 5'-ATTAACTCGGTTGATGTGACTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K121557-0022-057-H05C-02yp
CTTTTGCTGGACCTTCGGTTAGTTAACATCTGAATCAATATCCTTAACTGAATGATAGAG
GTATCAAATCTCGGATTTTCAAATATCATTGTAAGAAAGTATGTCACTTTGTTCCCTTTT
ACCTATGTGCATCATCACCTAACTTACTTTTGTTCTTTTCTCTGTTACTGTTTTTATTCT
CAAGCTTCGAGGACGGGCTGAAGCTTGTAAAACTTAGAGGAGAAGCTATGCAGGTAAGAT
TGAACTCTGCTCTGTATATCCTTCATTGACTATGTCAAGTTTAGGTTGATGTTGTGACGG
CTGAACTTTGTAGTGATCTTATATACCATTCGAACAGCTGCATTGACTTTTAGTTTTTGA
TAAAATGACTTTCTTCAGGCTGCTGCAGATGCTGCTAAGAGTGCCATGGTTAGTATCATA
GGGTTGGACTCAGAAAAGGTTCAGCAGTTGTGTGATGCAGCAAATCAAGAAGTAGATGAA
GCTGACAAAGTTCAGATCGCAAATTACTTATGTCCGGTATATATGTCTTCCATGAATCTT
TTGCATTCTAGACTTGTATAAAATCATTTTCACATTCCTTTATGTGGTATACTGTTACAG
GGTAATTACGCAGTATCTGGAGGTCTTAAAGGAATCGAAGTTGTTGAAGCCAAAGCTAAG
TCATTCAAAGCACGAATGACGGTATCCTCTCTCTCTAATTCCATTTATGTTATGAAATCT
TCCTCGATGCTATCAGAAACATGATTGTTGGCGGTTGGTTCTGTAGGTGCGTCTAGCTGT
TGCAGGTGCTTTCCACACTAGTTTCATGGAACCAGCAGTCTCGAGATTAGAAGCTGCATT
GGCAGCCACAGAGATCAGAAGTCCGAGGATCCCAGTGATCTCGAATGTCGATGCACAGCC
TCATGC
   
Sequence Name 67-K121556-0022-057-H05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K121556-0022-057-H05C-8409
ATTTACATTGAATTATCCTGCATATCCATCATATCCATTGAGTTTACGAAGATAGAAAAA
TACCTTTCCAGGTCCCAATTCGTAGCTGCTTTTAAGTCCTTTGGATAAGAGAGTCTTTAC
TGTTGTCTCCCATTGGACTGGAGATGTCACCTGTAGTCAACATTTGCATCTTTTTTTTAT
ATAGCGTCGCCAAAAGAGAAATAATTCACATAGTTCTCTGAGTATTGATTTATTACCTGG
CGTGCAAGTATCTTCTTGATCGTGTCTGGATCTGCATGAGGCTGTGCATCGACATTCGAG
ATCACTGGGATCCTCGGACTTCTGATCTCTGTGGCTGCCAATGCAGCTTCTAATCTCGAG
ACTGCTGGTTCCATGAAACTAGTGTGGAAAGCACCTGCAACAGCTAGACGCACCTACAGA
ACCAACCGCCAACAATCATGTTTCTGATAGCATCGAGGAAGATTTCATAACATAAATGGA
ATTAGAGAGAGAGGATACCGTCATTCGTGCTTTGAATGACTTAGCTTTGGCTTCAACAAC
TTCGATTCCTTTAAGACCTCCAGATACTGCGTAATTACCCTGTAACAGTATACCACATAA
AGGAATGTGAAAATGATTTTATACAAGTCTAGAATGCAAAAGATTCATGGAAGACATATA
TACCGGACATAAGTAATTTGCGATCTGAACTTTGTCAGCTTCATCTACTTCTTGATTTGC
TGCATCACACAACTGCTGAACCTTTTCTGAGTCCAACCCTATGATACTAACCATGGCACT
CTTAGCAGCATCTGCAGCAGCCTGAAGAAAGTCATTTTATCAAAAACTAAAAGTCAATGC
AGCTGTTCGAATGGTATATAAGATCACTACAAAGTTCAGCCGTCACAACATCAACCTAAA
ACTTGACATAGTCAATGAAGGATATACAGAGCAGAGTTCAATCTTACC

Last Updated on 06.12.2018 16:11