Sequences from confirmation amplicon


Confirmation sequences for line 057H08 and hit At1g13580 are shown.
Line ID 057H08
Locus At1g13580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4646521
Primers used for PCR 25mk - 8474, show primer details
   
Sequence Name 20-K114217-0022-057-H08C-25mk
GenBank Accession LN495304 [GenBank]
Sequencing primer gene specific primer, 25mk: 5'-TTCCTGATCTTCTTCTTTCTCTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K114217-0022-057-H08C-25mk
TTCATAGATCAATATTTTGCCAATTTCTACAAAAAAAACAATCATCAAGTCAAAGTTAGA
AAGTTAGTAACTTGACCCCAAAAAAAGAAGCTTTCTACAAACCCAACTTGAAAGTAACCA
AAGGCACACATAAATGATGAGGAACAAAAGAGAGAACTAGAGCTAAACAAATGTCAAGTA
CTCACTACATTGAAAAGAATGCAACACAATCAAGTGTCAAACAACATCACAAGACCTACA
TTCATTCACACAAACTTTAACATTTGTTATAAAGTATAAGAAAAGGTATAAATCATTCTT
CACATTTATCAAAAATAGATACTTTCAGCTACAATCATTCTCCTTAGTGAACCTTTTCTA
TACTTTTAGCTGAAGCAAACAATTAAACTAAGTAACTTAAGCAACACACAAGATACTATA
TAAATCTAACACATATATACTCAAATATGTGTTTGATCAGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 02-K114216-0022-057-H08C-8409
GenBank Accession LN487127 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K114216-0022-057-H08C-8409
TTTCATTGAATATATCCTGATCAAACACATATTTGAGTATATATGTGTTAGATTTATATA
GTATCTTGTGTGTTGCTTAAGTTACTTAGTTTAATTGTTTGCTTCAGCTAAAAGTATAGA
AAAGGTTCACTAAGGAGAATGATTGTAGCTGAAAGTATCTATTTTTGATAAATGTGAAGA
ATGATTTATACCTTTTCTTATACTTTATAACAAATGTTAAAGTTTGTGTGAATGAATGTA
GGTCTTGTGATGTTGTTTGACACTTGATTGTGTTGCATTCTTTTCAATGTAGTGAGTACT
TGACATTTGTTTAGCTCTAGTTCTCTCTTTTGTTCCTCATCATTTATGTGTGCCTTTGGT
TACTTTCAAGTTGGGTTTGTAGAAAGCTTCTTTTTTTGGGGTCAAGTTACTAACTTTCTA
ACTTTGACTTGATGATTGTTTTTTTTGTAGAAATTGGCAAAATATTTGATCTATGGAAAA
CATAGACAGGATATGGGAGATGACACAACCGAGAGAAAGAAGAGAATCAGGA

Last Updated on 06.12.2018 16:11