Sequences from confirmation amplicon


Confirmation sequences for line 057H09 and hit At4g02480 are shown.
Line ID 057H09
Locus At4g02480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1085613
Primers used for PCR 71gp - 8474, show primer details
   
Sequence Name 22-K111730-0022-057-H09C-71gp
GenBank Accession LN496174 [GenBank]
Sequencing primer gene specific primer, 71gp: 5'-ATGACCCTCCTTCACTCTCACTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K111730-0022-057-H09C-71gp
TAGCTACAGCCATAAGTAGTTATCACAAAACTTAAAGTAAAAGCTACCTCAAAGATTTCA
TTAACGGCGAGTTTATCAGCATCATCGCTAGAAGAACCTTCTAACCGAAGTGAGCTAGCT
GCACACAAAGAATAATATTAATAAAGTTCACTTTTTAAGTTAAGTAAAGTTAGAAAGAAA
AAAAACAGCCCGCGATTACCAGCACAAAAAAAACCATGGTCTTCTTCGCAGAGGCCACCA
AGATCATTGCCATCTTGTACAGGCCTATCGAATCTAATCCCAATTTTTGAAGCACAGTTG
TCTTCAAATGCAAGGGCTACTTTTCCCTGGGAACCAATGGCCGGTCCCCTATATTACATA
GCAGGGAAAAAGTAAGAGCAAAGTTGTGAATACAACACACATTTACAGCAGATGTGAAGA
CTTTTAAAAACAGTAAACAATACCTAAGCTGGCCTTGAAGAGAAGAAATTGCAGAAGCCG
AAGGACCTACAAATTTAACTCTGTCACCTGAAAATGATAAAAATGCCCAAAAAAATTACC
AATTCAAATGTCAATATAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCG
   
Sequence Name 06-K111729-0022-057-H09C-8409
GenBank Accession LN488875 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K111729-0022-057-H09C-8409
TGACATTTGAATTGGTAATTTTTTTGGGCATTTTTATCATTTTCAGGTGACAGAGTTAAA
TTTGTAGGTCCTTCGGCTTCTGCAATTTCTTCTCTTCAAGGCCAGCTTAGGTATTGTTTA
CTGTTTTTAAAAGTCTTCACATCTGCTGTAAATGTGTGTTGTATTCACAACTTTGCTCTT
ACTTTTTCCCTGCTATGTAATATAGGGGACCGGCCATTGGTTCCCAGGGAAAAGTAGCCC
TTGCATTTGAAGACAACTGTGCTTCAAAAATTGGGATTAGATTCGATAGGCCTGTACAAG
ATGGCAATGATCTTGGTGGCCTCTGCGAAGAAGACCATGGTTTTTTTTGTGCTGGTAATC
GCGGGCTGTTTTTTTTCTTTCTAACTTTACTTAACTTAAAAAGTGAACTTTATTAATATT
ATTCTTTGTGTGCAGCTAGCTCACTTCGGTTAGAAGGTTCTTCTAGCGATGATGCTGATA
AACTCGCCGTTAATGAAATCTTTGAGGTAGCTTTTACTTTAAGTTTTGTGATAACTACTT
ATGGCTTGTAGCTACAGGATAGTAACCTCTTGACCCATACCATAGGTCGCACTTAGTGAG
AGTGAAGAAGGGTC

Last Updated on 06.12.2018 16:11