Sequences from confirmation amplicon


Confirmation sequences for line 058B05 and hit At5g56670 are shown.
Line ID 058B05
Locus At5g56670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22935595
Primers used for PCR 02rq - 8474, show primer details
   
Sequence Name 51-K116102-0022-058-B05C-02rq
GenBank Accession LN507606 [GenBank]
Sequencing primer gene specific primer, 02rq: 5'-GTTCATCGGTTATTGGTTCAGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K116102-0022-058-B05C-02rq
ATCAGTGTTATCGTACAGATCCTTGCAAGTGTTATAGAATGATGAAAATGTATTTAATAG
CTAATTTTCAATTGGCAAATTATCAACTTTTAATAGTCTGTGTAAAAGTATTGACAGAAA
CTTAGAACACGAATTATGATCATTGGTCTTGTTGTAATGTATTGGCAAAAATCATATCTT
ATGTTTAATTGACCAAAATCATAAGTGAAAAAACAGTATTTCATAGCATAGAATCAAAAC
ATAATAGTTCGGTGCCAAAGAAAACAAAAAGTTACAAATGACCTTGAGAAGTTTCACGAC
AACATCTTTCTCTCTAACTTCTTCTATTTCTCAGAAGAGTTGGGTCCTCTCTTCTTTCCG
AAACCAACAACTGCACAAATCAATGAACCATTACTAACCAATAATAGATACGCAACGTAA
TAAGCATTTGAGTCAAAACCAAATGATAAATTCAGAAGCTCTAGTCTAAAACATCGAACC
ACAAGTGTTCCAATATAACACATTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 33-K116101-0022-058-B05C-8409
GenBank Accession LN501133 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K116101-0022-058-B05C-8409
TTGAATAATCCTGAAATGTGTTATATTGGAACACTTGTGGTTCGATGTTTTAGACTAGAG
CTTCTGAATTTATCATTTGGTTTTGACTCAAATGCTTATTACGTTGCGTATCTATTATTG
GTTAGTAATGGTTCATTGATTTGTGCAGTTGTTGGTTTCGGAAAGAAGAGAGGACCCAAC
TCTTCTGAGAAATAGAAGAAGTTAGAGAGAAAGATGTTGTCGTGAAACTTCTCAAGGTCA
TTTGTAACTTTTTGTTTTCTTTGGCACCGAACTATTATGTTTTGATTCTATGCTATGAAA
TACTGTTTTTTCACTTATGATTTTGGTCAATTAAACATAAGATATGATTTTTGCCAATAC
ATTACAACAAGACCAATGATCATAATTCGTGTTCTAAGTTTCTGTCAATACTTTTACACA
GACTATTAAAAGTTGATAATTTGCCAATTGAAAATTAGCTATTAAATACATTTTCATCAT
TCTATAACACTTGCAAGGATCTTGTACGATAACACTTGATTAATTCAATGGAGTGTTAGG
TTTGTGCTTCTGAATCTGAACCA

Last Updated on 06.12.2018 16:11