Sequences from confirmation amplicon


Confirmation sequences for line 058B11 and hit At5g18255 are shown.
Line ID 058B11
Locus At5g18255 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6035646
Primers used for PCR 02re - 8474, show primer details
   
Sequence Name 79-K115990-0022-058-B11C-02re
GenBank Accession LN513871 [GenBank]
Sequencing primer gene specific primer, 02re: 5'-TTTCTCGAGTGTCTGGGAAACTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K115990-0022-058-B11C-02re
ATCCAAATCTATATTTCAGGCTACAAGAAACTCTAGTTAACTAGGAACAACAAGCCTTAC
ACACGAATTAAGTTTTCTTCTAGTTGATAAGAACAAAACAAAACAAAAACAGAAAACCAA
AACCATAATCCACAAATTCGAATCGAACAAAAACAAAAAGCTAGAAGAAGAAACCTTAAA
TAATCAAAATCTAAAATTTTCAAAAACACATAAATATTTGGTTGGGTGGGTGTTAAAGCT
CGCAATAGCCATCACAGCGCCGAGTAAAATCGTGCGCTCAGGCAGCAAGCAGTCTTCATC
GCTGAAAACAATGGGAAATTAACGATAAAAGGAATCGAAACCTTTCCGTCACCGGAGCCG
CCAGCCAAGGATATTGGGGTTGCTCATTGGCCCGAAAAGGCCGAAACCCTAACGATAAAT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGC
   
Sequence Name 60-K115989-0022-058-B11C-8409
GenBank Accession LN510344 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K115989-0022-058-B11C-8409
CATTTACATTGATTTATCGTTAGGGTTTCGGCCTTTTCGGGCCAATGAGCAACCCCAATA
TCCTTGGCTGGCGGCTCCGGTGACGGAAAGGTTTCGATTCCTTTTATCGTTAATTTCCCA
TTGTTTTCAGCGATGAAGACTGCTTGCTGCCTGAGCGCACGATTTTACTCGGCGCTGTGA
TGGCTATTGCGAGCTTTAACACCCACCCAACCAAATATTTATGTGTTTTTGAAAATTTTA
GATTTTGATTATTTAAGGTTTCTTCTTCTAGCTTTTTGTTTTTGTTCGATTCGAATTTGT
GGATTATGGTTTTGGTTTTCTGTTTTTGTTTTGTTTTGTTCTTATCAACTAGAAGAAAAC
TTAATTCGTGTGTAAGGCTTGTTGTTCCTAGTTAACTAGAGTTTCTTTGTAGCCTGAAAT
ATAGATTTTGGATTACAAGTTAACTAAAATGCGTACTGCTTCTGTCTATAGTTTCCCAGA
CCTCCGAGAAAAAA

Last Updated on 06.12.2018 16:11