Sequences from confirmation amplicon


Confirmation sequences for line 058E07 and hit At3g49601 are shown.
Line ID 058E07
Locus At3g49601 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18387839
Primers used for PCR 11xy - 8474, show primer details
   
Sequence Name 31-K119596-0022-058-E07C-11xy
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 11xy: 5'-TGAAAGATGTACAACGGAATAGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K119596-0022-058-E07C-11xy
GTTCGACGAATAGTTTTTTGTGAGACCTAGAAATGGTGGTAAGCCTGTTAAAGGTGGGAA
AGGTTTTGAGGATGATGAAGGTACTGCTGGTTTATCTAAGAAACCCAATAAAGCTATCCT
TGAGCATGACCGTAAACGTCAGATTCATCTTAAGCTTGCTATTCTTGAGGATAAGCTTGC
TGATCAGGGTTATTCTGATATTGAGATTGCTCAGAAGCTTGAGGAAGCTAGAGTGAGTCT
TGAAGCTGCTGCTGCTGCTAATGAAGAAGAGAGTGATTCGAAGGTTTCTAATACTCAGAC
TCATCAGGTTGCTGCTAGGAAAGAGAAGCAGATGGAAGCGTTTCGAGCTGCGTTGGGTTT
GCCTGATCAGCAACAGGTGGCTGAGGAGGGTATTATTGATGATGAGCCGATGAGGGAGGG
TTTTGAAGGTAGATTGAAAGAGAGGCGGGAACATTCTTTTTTGGACAGAGATAGTGGGAG
GAAGAAGGTAGATGAGGATGTGGATGAGAAAGATGCTAAGGTAAAGGAAAGCAAGAAGCA
GCGAGGTGGTGATGATGATGATGTGGATGTAGTGAAGCGTCATAAGAAAAAAGAGAGCAA
GAAGAGAAGACATGATGATTCATCTGAATCTGATGAGCATGGTCGTGACCGTAGAAGGCG
CTCTAAGAAGAAGGCTAAGGGGCGTAAACAAGAAAGTGAAAGTGACTCTAGCAGCTCGGA
TTCTGAATCTGACTCTGACAGTGATGATGGAAAGAAGAGAGGAAGGAAGAAGCCTACAAA
GACTACAAAGAAAACCAGCAGAAGGAAAAGAATTGTCTCTTCTGAATCT
   
Sequence Name 07-K119595-0022-058-E07C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K119595-0022-058-E07C-8409
ATTTCCCCTCATCCTCCTCTCTTTTGTGTTACGTCCTCGTTGGTAACTTTCATTCTTTAG
CTTCTTCCTATTTTCATACTCAGCCTCACTGTCATCAGAGTCTCTAGCAACTTCCTTGCC
ACGAGAATCACTTCTATACTTGTCCTTGCCATGTTTGGTCACATCTTCTTCCTCTTTTCT
TTTCTGGCTCATTCCTGCACTGTAAACTTCTACCTTACTGCGCAGTTGTTTCTTGTTCTG
ATACTCAATCTCACTGTCATCAGAGTCTCTTGCAGCTTTCTTGTCATCTCTAGTATATCT
GTCTTTCAAATGATCAGCCTCCACGTCTTCATCATCTCTTTTCTGCTTCTGTCCTCCACG
GTAAGCTTCTTCTTTCTTTCGCAGTGGTTGTTTATTATCCTCCGATTCAGGCTCGCTAAC
ATCAGAATCATGCCTCTTTCGTCCAGCTCGGCTTTGTTCATCATGTTTGTCCCTTAATTC
TTTGGAACCAGATCGGTTGGAGGGGAGGCTCTTTTTGTGTGACTTCCTAAGTTTCTTGCT
ATCATCACTCTCAACTTCTTCAGATTCAGAAGAGACACTTCTTTTCCTTCTGCTGCGTTT
CTTTGTAGTCTTTGTAGGCTTCTTCCTTCCTCTCTTCTTTCCATCATCACTGTCAGAGTC
AGATTCAGAATCCGAGCTGCTAGAGTCACTTTCACTTTCTTGTTTACGCCCCTTAGCCTT
CTTCTTAGAGCGCCTTCTACGGTCACGACCATGCTCATCAGATTCAGATGAATCATCATG
TCTTCTCTTCTTGCTCTCTTTTTTCTTATGACGCTTCACTACATCCACATCATCATCATC
ACCACCTCGCTGCTTCTTGCTTTCTTTTACTTTAGCATCTTTCTCATCCACATCCTCATC
TACCTTCTTCCTCCCACTATCTCTGTCCAAAAA

Last Updated on 06.12.2018 16:11