Sequences from confirmation amplicon


Confirmation sequences for line 058F03 and hit At4g27940 are shown.
Line ID 058F03
Locus At4g27940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13904748
Primers used for PCR 21af - 8474, show primer details
   
Sequence Name 57-K113393-0022-058-F03C-8409
GenBank Accession LN509478 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K113393-0022-058-F03C-8409
GCATTTCATTGAATTATCATTGTTGAGGCAGAGCGCGCGGAGAATAGTTGGATTGCTTCG
GAAACGTCTCCGTCGTCGTCTCCGGTGGTAATAGAAAGCAGTGATTTTTCGATAACTGAT
GGAATGAGAGATGTGAGCGGATTTGGTATAGCTTCTCGACCTCAGTTGGACAAGGGTTTG
TCGGAAAACAATATAGGGTTCACGGAAAGAGTTTTCTCTGCCGCCGGAGCTGCGGTTTTG
TCCGCCGTTACTCTAAATCCTCTTGACGTTGTCAAGGTAGGAGCTTTCTATTGTTGTCTT
GATTCTCNTGGGAAA
   
Sequence Name 76-K113394-0022-058-F03C-21af
GenBank Accession LN513432 [GenBank]
Sequencing primer gene specific primer, 21af: 5'-ATTTCCCAGATGAATCAAGACAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K113394-0022-058-F03C-21af
CGTCAGAGGATTAGAGTAACGGCGGACAAAACCGCAGCTCCGGCGGCAGAGAAAACTCTT
TCCGTGAACCCTATATTGTTTTCCGACAAACCCTTGTCCAACTGAGGTCGAGAAGCTATA
CCAAATCCGCTCACATCTCTCATTCCATCAGTTATCGAAAAATCACTGCTTTCTATTACC
ACCGGAGACGACGACGGAGACGTTTCCGAAGCAATCCAACTATTCTCCGCGCGCTCTGCC
TCAACAATGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATTA

Last Updated on 06.12.2018 16:11