Sequences from confirmation amplicon


Confirmation sequences for line 058G03 and hit At1g34520 are shown.
Line ID 058G03
Locus At1g34520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12623542
Primers used for PCR 38of - 8474, show primer details
   
Sequence Name 62-K114722-0022-058-G03C-38of
GenBank Accession LN510819 [GenBank]
Sequencing primer gene specific primer, 38of: 5'-ATGTTGTGGATGATAAGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K114722-0022-058-G03C-38of
GTACAATAAAATAAAGGATGACAATACACATTCTGTTGTGCATGTAAGTTTTCTTTATCA
ATAAAACAATAAACAAACTTGTGATTAAAAATTATGGCTTACTTATCCTTAAACATTTCT
CCAGGTTGTCCGTATATGAAAGAATTGTGTGAAAATATGATACGAGTCATATGAATACAA
TCAACAGGCTGTTAGTGAATTGAAATAGATTTTGTACAATAAGACAAAAGATTTTATTTC
GATCTACTTAAGATAAAAGTGTTTGTTTATTTGGAAGCTACAACCCCATGAATCCGATGC
CAGCGATGTAGTATTTGGAGTGATCTCCTATTAGATTGCGTTAAAAAAAAATATATATTA
TTTTTCTTCAATCGATAACGTTTTGAACACGTCAAACAACCACCAAGTTAAAACTAGGTT
ATTGCAGAAACTGAGCAACGCAACAGAAAAAGAAAAACAAAATGGAGGAAGAACTCAAGA
GCTTCGTCAAAGTATGGGGTTCTGCAATAATCTCTGTCTCTTACTGTCACAGACAGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
   
Sequence Name 50-K114721-0022-058-G03C-oriL
GenBank Accession LN507258 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K114721-0022-058-G03C-oriL
ATATCCTGTCTGTGACAGTAAGAGACAGAGATTATTGCAGAACCCCATACTTTGACGAAG
CTCTTGAGTTCTTCCTCCATTTTGTTTTTCTTTTTCTGTTGCGTTGCTCAGTTTCTGCAA
TAACCTAGTTTTAACTTGGTGGTTGTTTGACGTGTTCAAAACGTTATCGATTGAAGAAAA
ATAATATATATTTTTTTTTAACGCAATCTAATAGGAGATCACTCCAAATACTACATCGCT
GGCATCGGATTCATGGGGTTGTAGCTTCCAAATAAACAAACACTTTTATCTTAAGTAGAT
CGAAATAAAATCTTTTGTCTTATTGTACAAAATCTATTTCAATTCACTAACAGCCTGTTG
ATTGTATTCATATGACTCGTATCATATTTTCACACAATTCTTTCATATACGGACAACCTG
GAGAAATGTTTAAGGATAAGTAAGCCATAATTTTTAATCACAAGTTTGTTTATTGTTTTA
TTGATAAAGAAAACTTACATGCACAACAGATGTGTATTGTCATCCTTTATTTTATTGTAC
CACATGTGAAAGTGGAGATCCACGATGGTTGTTATAAGCTGCTACGAAATATGAGACACC
CTT

Last Updated on 06.12.2018 16:11