Sequences from confirmation amplicon


Confirmation sequences for line 058G12 and hit At4g28680 are shown.
Line ID 058G12
Locus At4g28680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14157305
Primers used for PCR 07rn - 8474, show primer details
   
Sequence Name 74-K115963-0022-058-G12C-07rn
GenBank Accession LN513135 [GenBank]
Sequencing primer gene specific primer, 07rn: 5'-ACTGCATCAACCAAATTTTTAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K115963-0022-058-G12C-07rn
GACATTTGAATTTTGGTTGCACAAAACATAGACATTTGAATAAATAAATTTAATTCGGTT
ATGTGACTAATTAAGACTATATAGTACATTACATGTCATTGTAAAAAAAAGTTGTACTAT
GTTGTGTAGAAATATGGGATATGGTTGCATGTGGATGCAGCTTATGCAGGGAATGCATGT
ATATGTCCAGAATATCGAAAATTTATTGACGGGATTGAAAACGCAGACTCCTTTAACATG
AATGCTCATAAATGGTTATTTGCTAATCAAACTTGTTCACCTCTTTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 74-K115962-0022-058-G12C-8409
GenBank Accession LN513134 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K115962-0022-058-G12C-8409
TACAAGAGGTGAACAAGTTTGATTAGCAAATAACCATTTATGAGCATTCATGTTAAAGGA
GTCTGCGTTTTCAATCCCGTCAATAAATTTTCGATATTCTGGACATATACATGCATTCCC
TGCATAAGCTGCATCCACATGCAACCATATCCCATATTTCTACACAACATAGTACAACTT
TTTTTTACAATGACATGTAATGTACTATATAGTCTTAATTAGTCACATAACCGAATTAAA
TTTATTTATTCAAATGTCTATGTTTTGTGCACCAAATTCAAATGTCTAATAATTGCAGTG
GTTGAAGCAGTGTGATCAATACGGTGGATGAAGTTGGTTAAAAATTTGTTTGATGCAGT

Last Updated on 06.12.2018 16:11