SimpleSearch - Line and FST details


Line specific information

 
Line ID 058G12
Vector Used pAC161
Line Availability available as T3 set from NASC (N405556)
Segregation Analysis 50:43:33
Confirmed for Hit At4g28680
Parent of DUPLO pair 12795
Parent of pair(s) none
Note

Gene hit At4g18150

 
Sequence (A. th genome BLAST matches underlined) 
>95-K016086-022-058-G12-8409
GATCATGAAATTTCCCGGACATGAAGCCTTTACAATTGAATATATCCTGATTATAATCTT
GTGTTATCTGACTTTCATATATTTGTCTGTTATGTTCAAACTACAGACCTTGCAGACGCC
TGGACGCGGATATGTCCCGAATCGCAGGAGCTATGGGATCCTCNCCTATAGGGAGACCCT
CGGGAGTGCTTGGCATTCCGTGCGATAATGACTTCTGTTCAACCACCCAAACGTCGGAAA
GCCTGACGACGGAGCAGCATTCCAAAAAGATCCCTTGGCTCGTCTGGGTCGGCTATGGGA
TCCTCCTTATAGTGAG
GenBank Accession FR801944 [GenBank]
Graphic View Graphic view of gene At4g18150
Predicted Position of Insertion Chr4:10050853 - go to primer design
BLAST e Value 7e-12
Hit Clone Code (BAC ID) F15J5
Hit Gene Code At4g18150 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation Serine/Threonine-kinase, putative (DUF1296)
Insertion Classification CDSi
Confirmation Status failed

Gene hit At4g28680

 
Sequence (A. th genome BLAST matches underlined) 
>95-K016087-022-058-G12-8409
TGGGATCCTCCCTATAGTGAGAGAGTCTACATGGATCAATCTTCTTTATATTAATTTTCG
AAATTCTGGCGCTATACATGCATTTCATGAATACCCAGGGTCCCCTCGCTACCATATAGA
ATATTTCTACACAACATAGTACAACTTTTTTTTACAATGACATGTAATGTACTATATAGT
CTTAATTAGTCACATAACCGAATTAAATTTATTTATTCAAATGTCTATGTTTTGTGCACC
AAATTCAAATGTCTAATAATTGCAGTGGTTGAAGCAGTGTGATCAATACGGAGGATGAAG
ATGGCTAAAAATTTGGTTGATGCAGCCCAATATTTATTCGATATGTGTTTAATTACCTTT
GCGATGNTCCCCAATGGAACCAAAGGATCAACGGCTGCTGAAGACGCTAGGCAAACCCTG
AAAACCCNGAAACCTTTATTATTTAACANTGGACAAAATAAANTTATGAAGATGGAATAC
AAATATGTGTGTAAATGCTCTTATTGTCNCAACTCAAATGTGGTTACTTA
GenBank Accession AL936783 [GenBank]
Graphic View Graphic view of gene At4g28680
Predicted Position of Insertion Chr4:14157281 - go to primer design
BLAST e Value 7e-132
Hit Clone Code (BAC ID) T5F17
Hit Gene Code At4g28680 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation L-tyrosine decarboxylase
Insertion Classification CDSi
Confirmation Status confirmed, show confirmation sequences
Primer and wt-amplicons show primer details


Last Updated on 10.06.2021 13:37