Sequences from confirmation amplicon


Confirmation sequences for line 059B03 and hit At3g53880 are shown.
Line ID 059B03
Locus At3g53880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19955031
Primers used for PCR 02qp - 8474, show primer details
   
Sequence Name 51-K115691-0022-059-B03C-02qp
GenBank Accession LN507600 [GenBank]
Sequencing primer gene specific primer, 02qp: 5'-TTTAAGCCTGAGAACATTATGCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K115691-0022-059-B03C-02qp
GTTGATTCGGCAAGGCAAGAGCCATTGGCGTAAGTAATTTCTCTACAAAGAAACTCTCAG
ATCTTGTGGAGGCTGCTCGTGTTCCTCCCGCTGTAAACCAGGTGGAATGTCATCCTTCAT
GGCAACAACATAAGCTACATGAGTTTTGCAAGTCCAAAGGGATCCACCTCTCAGTAAGTT
TTAAAACACTAAACATTTCCAAATCATTTAATGAGTCCGAATGTCATATTTATATACACT
TCGTGAATGTTGATTGTGGCAGGGATATTCGCCATTGGGATCTCCTGGAACAACGTGGGT
GAAGGCCGATGTTTTAAAGAGTCCGGTTATTGAAATGATTGCTAAAGAAATCGGGAAGTC
TCCCGCACAAACCGCGCTTCGATGGGGACTTCAAATGGGTCATAGTATACTTCCCAAAAG
CACAAATGAAGGAAGAATCAGAGAGAACTTTGATGTCTTGGGATGGTCTATTCCAAAAGA
AATGTTTGACAAGTTTTCCAAGATAGAGCAGGTAAGTGAAACAATAAACCACATTTCTGC
AAATTTCGTATCTGTGAGTAGTATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 03-K115690-0022-059-B03C-8409
GenBank Accession LN487621 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K115690-0022-059-B03C-8409
TCACAGATACCAAATTTGCAGAAATGTGGTTTATTGTTTCACTTACCTGCTCTATCTTGG
AAAACTTGTCAAACATTTCTTTTGGAATAGACCATCCCAAGACATCAAAGTTCTCTCTGA
TTCTTCCTTCATTTGTGCTTTTGGGAAGTATACTATGACCCATTTGAAGTCCCCATCGAA
GCGCGGTTTGTGCGGGAGACTTCCCGATTTCTTTAGCAATCATTTCAATAACCGGACTCT
TTAAAACATCGGCCTTCACCCACGTTGTTCCAGGAGATCCCAATGGCGAATATCCCTGCC
ACAATCAACATTCACGAAGTGTATATAAATATGACATTCGGACTCATTAAATGATTTGGA
AATGTTTAGTGTTTTAAAACTTACTGAGAGGTGGATCCCTTTGGACTTGCAAAACTCATG
TAGCTTATGTTGTTGCCATGAAGGATGACATTCCACCTGGTTTACAGCGGGAGGAACACG
AGCAGCCTCCACAAGATCTGAGAGTTTCTTTGTAGAGAAATTACTTACGCCAATGGCTCT
TGCCTTGCCTGAATCAACCAGTGCTTCCATTGCTTTCCATGTGCTCGGAATATCGATAGG
CATAA

Last Updated on 06.12.2018 16:11