Sequences from confirmation amplicon


Confirmation sequences for line 060B02 and hit At5g21010 are shown.
Line ID 060B02
Locus At5g21010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7137582
Primers used for PCR 25oh - 8474, show primer details
   
Sequence Name 87-K114716-0022-060-B02C-25oh
GenBank Accession LN514791 [GenBank]
Sequencing primer gene specific primer, 25oh: 5'-TCAAATGTATGAGCTGTTGCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K114716-0022-060-B02C-25oh
GAATTGTAGCAAGCCTGGATGGAACAGAGCATTAATACAAGCACGATCTAAACTAACAGA
AATAACTAAAGAATAACCACCAATGATCAATGATGTACCTTAAAAACCTTAGGTTCCAAA
TCGTTAATAGTAACCTCTGTATTGTTTGCTTCAAACTCACTGAAAAACTTAGACTTGAAA
AATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 39-K114715-0022-060-B02C-oriL
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K114715-0022-060-B02C-oriL
CAAAATCCATTCGGCGGAACCCTTTTTTGTACCACCATGAAATTTTTATACAATAATTTT
GGGCCCC

Last Updated on 06.12.2018 16:11