SimpleSearch - Line and FST details
Line specific information
| Line ID | 060B02 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N405678) |
| Segregation Analysis | 50:50:41 |
| Confirmed for Hit | At5g21010 At2g03875 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | 83949, 83951, 83954, 83956, 83958 |
| Note | |
Gene hit At3g06365
| Sequence (A. th genome BLAST matches underlined) | >10-K114296-0022-060-B02-8409 GTTGATTGCTTTTCGCCTATTGAAGGACGCGATCGCGAATTTGTCTCTTTCTCACCATCC ACGTTCACTATTATAATAACGCTGCGGACATCTACATTTTTGAATTGAAAAAAAATTGGT AATTACTCTTTCTTTTTCTCCATATTGACCATCATACTCATTGATGATCCATGTAGATTT CCCGGACATGAAGCCATTTACAATTGAATATATCCNATAGGGAACGTGATCTGGGTTTAG ACCGTCGTGAGACACGTTAGGTGTACCCAACTGATACCCTCCTCGCGATATTCATTCATC CCCCCCTGCCC |
| GenBank Accession | HE664205 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr3:14202780 - go to primer design |
| BLAST e Value | 1e-14 |
| Hit Clone Code (BAC ID) | F21A14 |
| Hit Gene Code | At3g06365 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | novel transcribed region |
| Insertion Classification | TS2TE |
| Confirmation Status | failed |
Gene hit At5g21010
| Sequence (A. th genome BLAST matches underlined) | >02-K106284-0022-060-B02C-8409 CCGTAAAAGGTGGATCCTGCAAAGCGTCTTACGTTAGAGCGCTTAACGTTGTCAGAAATA AGCGAAAACATTGATTGTAAAAGTTTTAGCCGTATTCCGATAATTAATTGTAGGCCGGGG TCGTGCGCCGGTTCGCCGTGTTAGTTCTTAAACCTGAAACTTACTCATTTCCGTCCATAG AATGTGTTAACGATAGAGACACCCCTAGGTAGACGCCCCCATATGCCGTATACGCGTAGT AAGGTGCAGCACGGAAAAAATGCCGAAAAGTATAGCAAATATTTTCTATGCAGCTGCGCT ACGCGCAACTCCGATCCTATTTTCACGATAGATATTCGCTATTCTCCCCCCTTTATTGCG TCAGGTTCATACCTTCCATCAAGTTCCAAAAACCTGTTTAAATATACTAGACCTCGAAAT TTCGCGCGAATATTGTCTATCACCTAGTGAGTAGTCTGTGACCCCTCCTAATATTTTAAC CAAACCGCGCATTCTTTCTCCAATGTTTAAATAGTATAATCACACCGTCAACACCCACAA CGTGTTCCCATCGCTTCACTCCTATGGCCCCCCCTTTTGACAGATTAAGTCATCAAC |
| GenBank Accession | HE664204 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr5:7137651 - go to primer design |
| BLAST e Value | 1e-07 |
| Hit Clone Code (BAC ID) | F22D1 |
| Hit Gene Code | At5g21010 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | BTB-POZ and MATH domain 5 |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
Gene hit At2g03875
| Sequence (A. th genome BLAST matches underlined) | >10-K114296-0022-060-B02-8409 GTTGATTGCTTTTCGCCTATTGAAGGACGCGATCGCGAATTTGTCTCTTTCTCACCATCC ACGTTCACTATTATAATAACGCTGCGGACATCTACATTTTTGAATTGAAAAAAAATTGGT AATTACTCTTTCTTTTTCTCCATATTGACCATCATACTCATTGATGATCCATGTAGATTT CCCGGACATGAAGCCATTTACAATTGAATATATCCNATAGGGAACGTGATCTGGGTTTAG ACCGTCGTGAGACACGTTAGGTGTACCCAACTGATACCCTCCTCGCGATATTCATTCATC CCCCCCTGCCC |
| GenBank Accession | HE664205 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr2:8809 - go to primer design |
| BLAST e Value | 1e-14 |
| Hit Clone Code (BAC ID) | F23H14 |
| Hit Gene Code | At2g03875 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | novel transcribed region |
| Insertion Classification | TS2TE |
| Confirmation Status | confirmed, show confirmation sequences |
Gene hit At4g00480
| Sequence (A. th genome BLAST matches underlined) | >10-K027724-022-060-B02-8409 TTTTAGTATATCTGAGCGCTGAGTATGGGATCCTCCCTATAGTGAGTCCTATTACTCACT CTCTTTTTATCCTCCCCTTCTTGACATGAAGTTTTACCACCACAATTAGCCGTTTAATTA CTTTTATTCCCGACTGTGTGGGTTATGCCTANTCANCCATGCTGATCTTCTTACTANCTC TTCTACTCTTGCCTCCAGTTCTTGCAGGTATGGNATCCTCCCTATAGTGAGN |
| GenBank Accession | CR770068 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr4:217380 - go to primer design |
| BLAST e Value | 2e-10 |
| Hit Clone Code (BAC ID) | F6N23 |
| Hit Gene Code | At4g00480 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | basic helix-loop-helix (bHLH) DNA-binding superfamily protein |
| Insertion Classification | CDSi |
| Confirmation Status | failed |
Gene hit At4g11320
| Sequence (A. th genome BLAST matches underlined) | >10-K027915-022-060-B02-8409 AAAATTTACTCATTGGACGTGTTCACACCAATGGGACACGCTTATAAATAACAATTTTAA TATATCTGAGCGCTGAGTATGGGATCCTCCCTATAGTGAGTCGTATTACTCACTCCCCTT TTCTATTTCCCCTGCTGACATCTCTGCTCCCCCCTATAATTATTCCTATTACTCACTTTT TTTTCCTCTCTTTGGGGGGGGGGGCGCTGTTCATATTTTTTTTTCATTGTATTTTTTATT ATTTTTATTTTTTCTTTTTGGAAGGGTTTGTGATGGGATCTGGCGGTTTGGTTCGTATTT GTAGGAGCGGTAGAAAATACGCGACGAAAGCTGATCTGTGCGCTGTGCGGTCCGGAGCAC TGTCGACGGGCACGCAGTGGGCCGGAGACGTAGNGCCTCTGGCGCCACGCGGGGGGGGGG GGCAAAAGAGAGTGTATTGA |
| GenBank Accession | CR770069 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr4:6887161 - go to primer design |
| BLAST e Value | 3e-16 |
| Hit Clone Code (BAC ID) | F8L21 |
| Hit Gene Code | At4g11320 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Papain family cysteine protease |
| Insertion Classification | Promoter |
| Confirmation Status | failed |
| Other FSTs Supporting this Hit | CR934490 [GenBank] CR770069 [GenBank] |
Gene hit MFJ20
| Sequence (A. th genome BLAST matches underlined) | >58-K013874-022-060-B02-8409 ATGGACATAAATAAAAGAGAAAAGTGTTGGCTATGGGATCCTCCCTATAGTGAGACGGAA AGGCAACTATTATAACATTTTTTTTTGCGCGTTAACCAGCTAAAAAAGGTTTTATTTACT GTGAAAAACAAAACAAAAGC |
| GenBank Accession | FR801964 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr3:10663110 - go to primer design |
| BLAST e Value | 2e-09 |
| Hit Clone Code (BAC ID) | MFJ20 |
| Insertion Classification | Genome Hit |
| Confirmation Status | failed |
|
Last Updated on 10.06.2021 13:37 |
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