Sequences from confirmation amplicon


Confirmation sequences for line 060D03 and hit At2g25355 are shown.
Line ID 060D03
Locus At2g25355 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10802237
Primers used for PCR 03et - 8474, show primer details
   
Sequence Name 20-K117874-0022-060-D03C-03et
GenBank Accession LN495399 [GenBank]
Sequencing primer gene specific primer, 03et: 5'-TGATGATGGTTCAGATTGAAGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K117874-0022-060-D03C-03et
TCTTCTGTTTGCTCCTTCAATGCAAGCACAGGCAAAAGAGCCAATTGTGGTCCTTTAATG
TCTATCCAATAGTTCTGCAATGAAACATGTGTCAAAACGATTTTCAAAATATTATGATCT
ACTTTGTTACATGCAACATGACTTTGGCTGAGAACTCTAGGGGCAACACTCTAAATAAAG
GCTTATGCCGTTTTCTTTATCCATGATCTCAAGTATTTATTAACATATACATGTAACTAA
CAAACAAGCTGAGCAATGATAGAAGTTGGATAATGATAGAGCACAACCGAAATCAATATT
CTTTATACATGATTTTTGCATTAAATGAACTGTATACAGAGAAAAAATGCTTGATTCAGA
GGGTCCAAGGGTAGAAAAAGAAAAAGGGCGTCCTCAAAAATGAAATGAGAACGTAGATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCC
   
Sequence Name 08-K117873-0022-060-D03C-8409
GenBank Accession LN489917 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K117873-0022-060-D03C-8409
CCCTTTTTCTTTTTCTACCCTTGGACCCTCTGAATCAAGCATTTTTTCTCTGTATACAGT
TCATTTAATGCAAAAATCATGTATAAAGAATATTGATTTCGGTTGTGCTCTATCATTATC
CAACTTCTATCATTGCTCAGCTTGTTTGTTAGTTACATGTATATGTTAATAAATACTTGA
GATCATGGATAAAGAAAACGGCATAAGCCTTTATTTAGAGTGTTGCCCCTAGAGTTCTCA
GCCAAAGTCATGTTGCATGTAACAAAGTAGATCATAATATTTTGAAAATCGTTTTGACAC
ATGTTTCATTGCAGAACTATTGGATAGACATTAAAGGACCACAATTGGCTCTTTTGCCTG
TGCTTGCATTTGAAGGAGCAAACAGAAGAAATTATCCCAAGTTTGAGGCAAGTACCTTCT
GAATATACTTCTTCAATCTGACCCATCATCAAAGAAAAGNAAGAGTCATTACCAATTTTT
TTTCAATTCAAAAATGTAAATATACCACACATTATT

Last Updated on 06.12.2018 16:11