SimpleSearch - Line and FST details


Line specific information

 
Line ID 060D03
Vector Used pAC161
Line Availability available as T3 set from NASC (N405703)
Segregation Analysis 50:43:37
Confirmed for Hit At2g25355
Parent of DUPLO pair 12274
Parent of pair(s) none
Note

Gene hit MFJ20

 
Sequence (A. th genome BLAST matches underlined) 
>68-K013874-022-060-D03-8409
GTAGATTGTCTGTTTATGGACATAAATACAAGAGAACGTGTTGGCTATGGGATCCTCCGT
ATAGTGAGATGCCTATAATGGGTTTNTTCCCAATTTTCTTCCAGATCAAAAATGTGGATG
TCCCGAGGGGTATTTTAATATGAAAGTACATTTGGAGAAAACGACAAATTACGATCCGAA
GAATTTATGGGCGAACGCTTTATTCTATTTATTCTAGTCCGGAAATCTCTATGTCGAGGG
GTGTACATTGCCGTGCAAAAGGGGGCCTAAATGAAAAAACTTCCGGGCGATGGCTTGCTT
TC
GenBank Accession FR801979 [GenBank]
Graphic View Graphic view of sequence FR801979 of line 060D03
Predicted Position of Insertion Chr3:10663106 - go to primer design
BLAST e Value 0.025
Hit Clone Code (BAC ID) MFJ20
Insertion Classification Genome Hit
Confirmation Status failed
Other FSTs Supporting this Hit FR801979 [GenBank]

Gene hit At2g25355

 
Sequence (A. th genome BLAST matches underlined) 
>20-K114296-0022-060-D03-8409
CAATAATCAAACTTATTTTTCTCTGTATACTGTCCATTTACTGCAAAAATCACGTATCGA
AAATATTCATTTCAGCTGTGCTCAATCTCTATCCAACTTCAATCAGTGCACATCTTGTTT
GTTAATTTCATGTATATGTGTATAAATAACCTGACATCTTCTCAAAATAGAACGGCATAC
TCCTTTATTTACAGTGTTGCCCCTAACCTGCCCTGCCACTACNCCCTTTTTCTTTTTCTN
CCCTTGTCCCCTCTGAATCACGCATTTTTTCTCTGTATACAGTTCATTTAATGCGCAAAT
CATGTATAAAGAATATTGATTTCCCTTGTGCTCTATCATTATC
GenBank Accession HE664221 [GenBank]
Graphic View Graphic view of gene At2g25355
Predicted Position of Insertion Chr2:10801979 - go to primer design
BLAST e Value 2e-34
Hit Clone Code (BAC ID) T22F11
Hit Gene Code At2g25355 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation PNAS-3-like protein
Insertion Classification CDSi
Confirmation Status confirmed, show confirmation sequences
Primer and wt-amplicons show primer details


Last Updated on 10.06.2021 13:37